Kong D L, Chen C Z, Lin E F, Yu Y T
Institute for Molecular Biology, Nankai University, Tianjin, China.
Artif Organs. 1998 Aug;22(8):644-50. doi: 10.1046/j.1525-1594.1998.06010.x.
A highly selective immunoadsorbent was prepared by immobilization of DNA on carbonized resin beads (Type I) for the removal of the pathogenic antibodies of systemic lupus erythematosus (SLE) patients. Thirty cases of clinical trials of this SLE therapy were performed at 12 hospitals in China. The levels of anti-DNA antibodies after whole blood perfusion were decreased 40-70%. Almost all the symptoms were relieved, and some patients were freed from medicine administration. A new immunoadsorbent was prepared using aminated cellulose beads (Type II) having a higher DNA immobilization capacity of 0.6 mg/ml than the 0.4 mg/ml capacity for Type I. Stationary adsorption tests with the sera of SLE patients showed that the Type II immunoadsorbent could remove 60% of the pathogenic antibodies, which is much higher than the 30% for the Type I adsorbent.
通过将DNA固定在碳化树脂珠（I型）上制备了一种高选择性免疫吸附剂，用于去除系统性红斑狼疮（SLE）患者的致病抗体。在中国12家医院对这种SLE治疗方法进行了30例临床试验。全血灌注后抗DNA抗体水平降低了40%-70%。几乎所有症状都得到缓解，一些患者不再需要服药。使用胺化纤维素珠（II型）制备了一种新的免疫吸附剂，其DNA固定能力为0.6 mg/ml，高于I型的0.4 mg/ml。用SLE患者血清进行的静态吸附试验表明，II型免疫吸附剂可去除60%的致病抗体，远高于I型吸附剂的30%。
