Afasizheva I Iu, Dolgikh D A, Abdullaev Z Kh, Latypov R F, Tiktopulo E I, Uverskiĭ V N, Ptitsyn O B, Kirpichnikov M P
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Biofizika. 1998 May-Jun;43(3):384-91.
A biologically active de novo protein albeferon was studied by physical techniques including CD spectroscopy in far and near ultraviolet regions and microcalorimetry. Albeferon was obtained by grafting an active octapeptide interferon fragment into a de novo protein albebetin used as a carrier. It was shown that attachment of the octapeptide into its molecule did not weaken albebetin but even slightly improved its structure and stability. The obtained results can be used for de novo protein design and improvement.
通过包括远紫外和近紫外区域的圆二色光谱法以及微量量热法在内的物理技术，对一种具有生物活性的从头合成蛋白质albferon进行了研究。Albferon是通过将一个活性八肽干扰素片段嫁接到用作载体的从头合成蛋白质albebetin中获得的。结果表明，八肽连接到其分子中并没有削弱albebetin，反而略微改善了其结构和稳定性。所得结果可用于从头合成蛋白质的设计和改进。
