[血小板激活和聚集过程中小角光散射的变化]
[Changes in small-angle light scattering by platelets during their activation and aggregation].
作者信息
Roshchupkin D I, Berzhitskaia V V, Sokolov A Iu
机构信息
Russian State Medical University, Moscow, Russia.
出版信息
Biofizika. 1998 May-Jun;43(3):503-10.
Small-angle light scattering (0.5-7 degrees) platelets is enhanced upon the adenosine-5'-diphosphate-induced formation of microaggregates of platelets. The intensity of the scattered light, measured within a short period of time after stimulation of platelets, may by used as a nephelometric quantitative index of their initial aggregation. The light transmittance of platelet suspensions decreases after stimulation due to both the rounding of cell body and the initial aggregation of cells. Sodium hypochlorite at concentrations corresponding to its content in tap water inhibits the Ca(2+)-induced aggregation of isolated rabbit platelets. The tap water causes the transition of platelets to a state that is refractory with respect to CaCl2.
在腺苷 - 5'-二磷酸诱导血小板形成微聚集体时，小角度光散射（0.5 - 7度）的血小板会增强。在刺激血小板后短时间内测量的散射光强度，可作为其初始聚集的比浊定量指标。由于细胞体变圆和细胞的初始聚集，刺激后血小板悬浮液的透光率会降低。与自来水中次氯酸钠含量相当的浓度的次氯酸钠会抑制离体兔血小板的钙（2 +）诱导聚集。自来水会使血小板转变为对氯化钙难治的状态。
Zotero 插件