[A protein of the annexin group in early fish development. I. Identification and biosynthesis of the protein in embryogenesis].

Ca-binding proteins were isolated from the loach (Misgurnus fossilis) eggs and embryos, using their capacity for binding to acidic phospholipids in the presence of Ca ions. It was shown that the major protein present in a mixture of these proteins was synthesized at the early developmental stages. Polyclonal rabbit antibodies were raised against this protein. These antibodies proved to be monospecific both to the protein of the loach eggs and embryos and that of the Brachydanio rerio embryos. The antibodies were used for screening cDNA library from the B. rerio embryos at 6 to 9 h of development. As a result of screening, a cDNA clone was obtained, which, when converted into a peptide sequence, shows a high degree of homology with proteins of the annexin group.