苏木精长时间染色对苏木精-伊红染色胃黏膜中检测幽门螺杆菌的影响。
Effect of prolonged staining with hematoxylin on detecting Helicobacter pylori in hematoxylin-eosin-stained gastric mucosa.
作者信息
Tazawa K, Tsutsumi Y
机构信息
Department of Pathology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
出版信息
Pathol Int. 1998 Jun;48(6):448-52. doi: 10.1111/j.1440-1827.1998.tb03931.x.
Helicobacter pylori (H. pylori) is identifiable in hematoxylin-eosin (HE)-stained preparations, particularly under a prolonged hematoxylin staining condition. The staining intensity of H. pylori bodies was increased when the hematoxylin staining period was doubled from 5 min, the routine period of Meyer's hematoxylin staining, to 10 min. The staining intensity of the background epithelial nuclei was only mildly increased. Hematoxylin-eosin staining employing a prolonged hematoxylin staining period gave detectability of H. pylori comparable to May-Giemsa and immunoperoxidase staining. The modified HE method is thus very useful and practical for identifying H. pylori in routine gastric biopsy specimens.
幽门螺杆菌（H. pylori）可在苏木精-伊红（HE）染色的标本中识别出来，尤其是在延长苏木精染色条件下。当苏木精染色时间从迈耶苏木精常规染色时间5分钟加倍至10分钟时，幽门螺杆菌菌体的染色强度增加。背景上皮细胞核的染色强度仅略有增加。采用延长苏木精染色时间的苏木精-伊红染色法对幽门螺杆菌的检测能力与梅-吉姆萨染色法和免疫过氧化物酶染色法相当。因此，改良的HE方法对于在常规胃活检标本中鉴定幽门螺杆菌非常有用且实用。
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