Gosse-Brun S, Sauvaigo S, Daver A, Larra F, Kwiatkowski F, Bignon Y J, Bernard-Gallon D
Laboratoire de Radioanalyse, Centre Paul Papin, Angers, France.
Anticancer Res. 1998 Jul-Aug;18(4A):2611-6.
The H-ras locus has been suggested to play an important role in susceptibility to cancer. However, the results remain controversial.
In order to elucidate the potential role of the H-ras locus in colorectal carcinogenesis, 142 colorectal tumors with matched normal samples were studied for genomic instability and loss of heterozygosity (LOH), and 143 healthy samples of white blood cell DNA were examined by PCR, for H-ras allelic polymorphism.
Nine percent of colorectal cancer patients constitutionally presented at least one rare allele versus 1.4% of healthy individuals (P = 0.0034). The risk of developing colorectal cancer increased significantly with the presence of rare H-ras alleles (odds ratio = 7.10 and 95% confidence interval = 1.92-26.35). The genotype associating one common allele and one rare allele was overrepresented in cancer patients (P < 0.01). No associations were observed between the rare alleles and tumor site or with the aggressiveness of cancer. Low frequencies of LOH (5%) and genetic instability (0.7%) at the H-ras locus were found in our colorectal cancer set.
Consequently, the presence of uncommon alleles at the H-ras locus appeared to be an informative genetic marker.
H-ras基因座被认为在癌症易感性中起重要作用。然而，结果仍存在争议。
为了阐明H-ras基因座在结直肠癌发生中的潜在作用，对142例结直肠癌及其配对的正常样本进行基因组不稳定性和杂合性缺失（LOH）研究，并通过PCR检测143例健康白细胞DNA样本的H-ras等位基因多态性。
9%的结直肠癌患者先天性存在至少一个罕见等位基因，而健康个体中这一比例为1.4%（P = 0.0034）。携带罕见H-ras等位基因时，患结直肠癌的风险显著增加（优势比 = 7.10，95%置信区间 = 1.92 - 26.
