Pocard M, Muleris M, Hamelin R, Salmon R J, Dutrillaux B, Poupon M F
Section de Recherche, Institut Curie, Paris, France.
Anticancer Res. 1998 Jul-Aug;18(4A):2743-7.
The ability of cancer cells to metastasize might depend on their reduced dependency on the originating tissue. To test this hypothesis, 10 human colorectal carcinomas (CRC) were implanted either onto nude mouse caecum wall (orthotopic site), or into the subcutaneous tissue (ectopic site), and their growth in these sites compared. Prognostic factors were studied: Astler-Coller modified Dukes's stage, loss of chromosome 17p and/or 18q, and their TP53 gene. Early stage CRC [B2 (n = 3) and C1 (n = 1)] were found to grow 1.7 to 3.6 times (p < 0.05, p < 0.008 respectively) more rapidly in the caecum than in subcutaneous tissue. Metastatic stage CRC [C1 (n = 1), C2 (n = 2) or D (n = 3)] grew similarly in both sites, and more slowly than those of the first group. No relationship was found between growth rates, TP53 mutations or karyotypes. Growth rate of non metastatic cancers was slowed down by implantation in a foreign tissue whereas growth of metastatic tumours was similar in both sites, indicating that they do not recognize or need tissue growth factors.
癌细胞转移的能力可能取决于它们对原发组织依赖性的降低。为了验证这一假设，将10例人类结肠直肠癌（CRC）分别植入裸鼠盲肠壁（原位部位）或皮下组织（异位部位），并比较它们在这些部位的生长情况。研究了预后因素：阿斯特勒-科勒改良的杜克分期、17号和/或18号染色体缺失以及它们的TP53基因。发现早期CRC [B2（n = 3）和C1（n = 1）]在盲肠中的生长速度分别比皮下组织快1.7至3.6倍（分别为p < 0.05，p < 0.008）。转移期CRC [C1（n = 1），C2（n = 2）或D（n = 3）]在两个部位生长相似，且比第一组生长缓慢。未发现生长速率、TP53突变或核型之间存在关联。非转移性癌症的生长速率因植入异体组织而减慢，而转移性肿瘤在两个部位的生长相似，表明它们不识别或不需要组织生长因子。
