Tsukui H, Pocard M, Salmon R J, Lefrançois D, Languille-Mimoune O, Dutrillaux B, Poupon M F
Département de Chirurgie, Université de Tokyo, Japan.
Gastroenterol Clin Biol. 1994;18(12):1112-8.
The xenograft of human cancers into athymic nude mice makes it possible to obtain abundant tumour material and deepen biological characterization. Usually, the tumours are grafted into the subcutaneous tissue whose environment is indeed different from that of original colon cancer.
In order to transplant tumours into an orthotopic environment, intra-caecal implantation of colon cancers was performed. A tumour fragment (30 mm3) was deposited at the surface of serous membrane of the caecum, previously scrapped, fixed with a thread and recovered with biologic glue. Six different types of colon tumour, previously adapted to transplantation in nude mice, were similarly grafted.
Twenty to forty days after transplantation, tumour takes were observed with a similar rate by the subcutaneous or intra-caecal graft (93% of 115 and 96.8% of 154 transplantations, respectively). Tumour growth varied between the tumours implanted into the two sites. Moreover, only the tumours intracaecally transplanted developed nodal and liver metastases. Histological examination revealed the invasion of the muscle layer, submucosal tissue and mucosal membrane by tumour cells and the mixture of normal glands and neoplastic glands.
This technique of orthotopic implantation of colon cancer samples will contribute to obtain an experimental model for colon cancer research.
将人类癌症异种移植到无胸腺裸鼠体内，使得获取大量肿瘤材料并深入进行生物学特性分析成为可能。通常，肿瘤被移植到皮下组织，其环境确实与原发性结肠癌的环境不同。
为了将肿瘤移植到原位环境中，进行了结肠癌的盲肠内植入。将一个肿瘤碎片（30立方毫米）放置在预先刮擦过的盲肠浆膜表面，用线固定并用生物胶覆盖。六种先前适应于在裸鼠体内移植的不同类型的结肠肿瘤也以同样的方式进行移植。
移植后20至40天，皮下或盲肠内移植的肿瘤发生率相似（分别为115次移植中的93%和154次移植中的96.8%）。植入两个部位的肿瘤生长情况有所不同。此外，只有盲肠内移植的肿瘤出现了淋巴结和肝转移。组织学检查显示肿瘤细胞侵袭了肌层、黏膜下层组织和黏膜，并且存在正常腺体和肿瘤腺体的混合。
这种结肠癌样本原位植入技术将有助于获得用于结肠癌研究的实验模型。
