通过高效液相色谱法同时检测高能磷酸盐以及糖酵解和三羧酸循环的代谢产物。
Simultaneous detection of high energy phosphates and metabolites of glycolysis and the Krebs cycle by HPLC.
作者信息
Vogt A M, Ackermann C, Noe T, Jensen D, Kübler W
机构信息
Department of Cardiology, University of Heidelberg, Germany.
出版信息
Biochem Biophys Res Commun. 1998 Jul 30;248(3):527-32. doi: 10.1006/bbrc.1998.9005.
PMID:9703959
Abstract
For the detailed analysis of energy metabolism, a HPLC method is described allowing the single-run separation and quantification of most metabolites from glycolysis and the Krebs cycle including the high energy phosphates. With a detection limit in the picomolar range this method is even applicable when only small sample sizes of tissue are obtained.
摘要
为了对能量代谢进行详细分析，本文描述了一种高效液相色谱（HPLC）方法，该方法能够单次分离并定量分析糖酵解和三羧酸循环中的大多数代谢物，包括高能磷酸盐。该方法的检测限在皮摩尔范围内，即使仅获得少量组织样本时也适用。
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