Kim Y J, Ahn J J, Woo B H
Department of Obstetrics and Gynecology, Ewha Womans University Hospital, Seoul, Korea.
Am J Obstet Gynecol. 1998 Jul;179(1):146-9. doi: 10.1016/s0002-9378(98)70265-0.
The purpose of this study was to determine the inhibitory effects of anti-interleukin-1beta and transforming growth factor-beta2 on the production of interleukin-1beta and prostaglandin E2 by human decidual cells.
Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1beta, and lipopolysaccharide plus transforming growth factor-beta2, respectively. Interleukin-1beta and prostaglandin E2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay.
At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1beta, anti-interleukin-1beta decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P < .05) and also decreased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P < .05). In the medium containing lipopolysaccharide plus transforming growth factor-beta2, transforming growth factor-beta2 also decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P < .05) but increased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentration of 1 ng/mL (P < .05).
Lipopolysaccharide-induced prostaglandin E2 production by human decidual cells in vitro may be prevented by anti-interleukin-1beta and transforming growth factor-beta2, and interleukin-1beta production may be prevented by anti-interleukin-1beta.
本研究旨在确定抗白细胞介素 - 1β和转化生长因子 - β2对人蜕膜细胞产生白细胞介素 - 1β和前列腺素E2的抑制作用。
从30例妊娠37至40周的患者获取蜕膜。分离蜕膜细胞并进行培养。分别用脂多糖作为对照、脂多糖加抗白细胞介素 - 1β以及脂多糖加转化生长因子 - β2进行孵育。通过酶联免疫吸附测定和放射免疫测定法检测白细胞介素 - 1β和前列腺素E2。
在脂多糖加抗白细胞介素 - 1β的24小时培养结束时，抗白细胞介素 - 1β在50 ng/mL、100 ng/mL和500 ng/mL浓度时显著降低脂多糖诱导的前列腺素E2产生（P <.05），并且在10 ng/mL、50 ng/mL和100 ng/mL浓度时也显著降低脂多糖诱导的白细胞介素 - 1β产生（P <.05）。在含有脂多糖加转化生长因子 - β2的培养基中，转化生长因子 - β2在1 ng/mL和10 ng/mL浓度时也显著降低脂多糖诱导的前列腺素E2产生（P <.05），但在1 ng/mL浓度时显著增加脂多糖诱导的白细胞介素 - 1β产生（P <.05）。
抗白细胞介素 - 1β和转化生长因子 - β2可抑制体外人蜕膜细胞由脂多糖诱导的前列腺素E2产生，抗白细胞介素 - 1β可抑制白细胞介素 - 1β产生。
