The effect of cytokine mediators on prostaglandin inhibition by human decidual cells.

OBJECTIVE

The purpose of this study was to determine the inhibitory effects of anti-interleukin-1beta and transforming growth factor-beta2 on the production of interleukin-1beta and prostaglandin E2 by human decidual cells.

STUDY DESIGN

Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control, lipopolysaccharide plus anti-interleukin-1beta, and lipopolysaccharide plus transforming growth factor-beta2, respectively. Interleukin-1beta and prostaglandin E2 were measured by enzyme-linked immunosorbent assay and radioimmunoassay.

RESULTS

At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1beta, anti-interleukin-1beta decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P < .05) and also decreased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P < .05). In the medium containing lipopolysaccharide plus transforming growth factor-beta2, transforming growth factor-beta2 also decreased the lipopolysaccharide-induced prostaglandin E2 production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P < .05) but increased the lipopolysaccharide-induced interleukin-1beta production significantly at the concentration of 1 ng/mL (P < .05).

CONCLUSIONS

Lipopolysaccharide-induced prostaglandin E2 production by human decidual cells in vitro may be prevented by anti-interleukin-1beta and transforming growth factor-beta2, and interleukin-1beta production may be prevented by anti-interleukin-1beta.