Hamano K, Ito H, Fujimura Y, Tsuboi H, Esato K
Yamaguchi University School of Medicine, First Department of Surgery, Ube, Japan.
Cardiovasc Surg. 1998 Jun;6(3):296-301. doi: 10.1016/s0967-2109(98)00006-4.
In order to examine the changes in coronary artery morphology following cardiac transplantation, the intimal and medial areas and vascular components were measured 30 and 60 days postoperatively in rats receiving both iso- (n = 5, each time) and allotransplantations (n = 7, each time). Although cyclosporine A was administered intramuscularly (10 mg/kg per day) for 20 days following transplantation, graft rejection progressed gradually in the allotransplanted group. There were no clear changes in the intimal or medial areas following transplantation in the isotransplanted group. The intimal area of the coronary arteries in the allograft group was significantly thicker 30 and 60 days following transplantation, while the medial area of the coronary arteries in this group was significantly thinner than that of the isotransplantation group at these same time points. In the thickened intimal areas in the allotransplantation group, only the density of smooth muscle cells was higher following transplantation. In the thinned medial areas in the allotransplantation group, the densities of the smooth muscle cells and collagen were less than those in the isotransplantation group. These changes suggest that following transplantation, smooth muscle cells proliferate in the intimal areas, while smooth muscle cells and collagen are degraded in the medial areas.
为了研究心脏移植后冠状动脉形态的变化，对接受同基因移植（每次n = 5）和同种异体移植（每次n = 7）的大鼠在术后30天和60天测量其内膜和中膜面积以及血管成分。尽管移植后20天内每天肌肉注射环孢素A（10 mg/kg），但同种异体移植组的移植排斥反应仍逐渐进展。同基因移植组移植后内膜或中膜面积无明显变化。同种异体移植组冠状动脉的内膜面积在移植后30天和60天显著增厚，而该组冠状动脉的中膜面积在相同时间点显著薄于同基因移植组。在同种异体移植组增厚的内膜区域，仅移植后平滑肌细胞密度较高。在同种异体移植组变薄的中膜区域，平滑肌细胞和胶原的密度低于同基因移植组。这些变化表明，移植后平滑肌细胞在内膜区域增殖，而中膜区域的平滑肌细胞和胶原降解。
