Marshall R L, Cockerill J, Friedman P, Hayden M, Hodges S, Holas C, Jennings C, Jou C K, Kratochvil J, Laffler T, Lewis N, Scheffel C, Traylor D, Wang L, Solomon N
Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
J Virol Methods. 1998 Jul;73(1):99-107. doi: 10.1016/s0166-0934(98)00050-0.
The recent publication of representative genomic sequences of GBV-C has permitted the selection of PCR primers for detection of GBV-C in clinical samples by PCR techniques. Traditional amplification methodologies which couple reverse transcription polymerase chain reaction (RT-PCR) and Southern blot detection are slow, cumbersome, and can be technique dependent. This has hampered studies to determine the clinical significance of GBV-C. We report the selection of highly conserved PCR primers and a probe useful for semi-automated RT-PCR using the Abbott LCx system. This adaptation of the LCx system expands its capabilities to include the detection of RNA by RT-PCR, in addition to DNA detection by ligase chain reaction (LCR).
