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通过散布重复元件（IRE）-PCR技术鉴定小鼠中的新型简单序列长度多态性（SSLP）。

Identification of novel simple sequence length polymorphisms (SSLPs) in mouse by interspersed repetitive element (IRE)-PCR.

作者信息

Detter J C, Nguyen Q A, Kingsmore S F

机构信息

Department of Medicine and Center for Mammalian Genetics, University of Florida, 1600 SW Archer Road,PO Box 100221, Gainesville, FL 32610, USA.

出版信息

Nucleic Acids Res. 1998 Sep 1;26(17):4091-2. doi: 10.1093/nar/26.17.4091.

DOI:10.1093/nar/26.17.4091

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147784/

Abstract

Interspersed repetitive element (IRE)-PCR is a useful method for identification of novel human or mouse sequence tagged sites (STSs) from contigs of genomic clones. We describe the use of IRE-PCR with mouse B1 repetitive element primers to generate novel, PCR amplifiable, simple sequence length polymorphisms (SSLPs) from yeast artificial chromosome (YAC) clones containing regions of mouse chromosomes 13 and 14. Forty-two IRE-PCR products were cloned and sequenced from eight YACs. Of these, 29 clones contained multiple simple sequence repeat units. PCR analysis with primers derived from unique sequences flanking the simple sequence repeat units in seven clones showed all to be polymorphic between various mouse strains. This novel approach to SSLP identification represents an efficient method for saturating a genomic interval with polymorphic genetic markers that may expedite the positional cloning of genes for traits and diseases.

摘要

散在重复元件（IRE）-聚合酶链反应（PCR）是从基因组克隆重叠群中鉴定新的人类或小鼠序列标签位点（STS）的一种有用方法。我们描述了使用带有小鼠B1重复元件引物的IRE-PCR，从包含小鼠13号和14号染色体区域的酵母人工染色体（YAC）克隆中生成新的、可通过PCR扩增的简单序列长度多态性（SSLP）。从8个YAC中克隆并测序了42个IRE-PCR产物。其中，29个克隆包含多个简单序列重复单元。用源自7个克隆中简单序列重复单元侧翼独特序列的引物进行PCR分析，结果表明在各种小鼠品系之间均具有多态性。这种鉴定SSLP的新方法代表了一种用多态性遗传标记饱和基因组区间的有效方法，这可能会加快对性状和疾病相关基因的定位克隆。