小鼠基因组的单链构象多态性（SSCP）图谱绘制：小鼠1号染色体的SSCP、微卫星和基因图谱整合

Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1.

作者信息

Hunter K W, Watson M L, Rochelle J, Ontiveros S, Munroe D, Seldin M F, Housman D E

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Genomics. 1993 Dec;18(3):510-9. doi: 10.1016/s0888-7543(11)80007-8.

DOI:10.1016/s0888-7543(11)80007-8

PMID:8307560

Abstract

Interspersed repetitive sequence (IRS) PCR and repetitive element-to-bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high-density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.

摘要

散布重复序列（IRS）聚合酶链反应（PCR）和重复元件到泡状结构（IRS-泡状结构）PCR已被用于从小鼠-仓鼠体细胞杂种中快速生成大量小鼠特异性的、富含1号染色体的序列标签位点（STSs）。单链构象多态性（SSCP）已被用于将39个新的与重复元件相连的STSs定位到小鼠图谱上：22个位于1号染色体，10个位于15号染色体，2个分别位于12号和14号染色体，3个位于7号染色体。此外，我们整合了小鼠1号染色体的SSCP、单链长度多态性和限制性片段长度多态性图谱，从而得到了该染色体的高密度图谱，其中包含100多个位点，所有这些位点均在单个种间回交中进行了分型。