Kitano T, Sumiyama K, Shiroishi T, Saitou N
Laboratory of Evolutionary Genetics, National Institute of Genetics, Mishima, Japan.
Biochem Biophys Res Commun. 1998 Aug 10;249(1):78-85. doi: 10.1006/bbrc.1998.9074.
We have sequenced the complete coding region of the Rh blood group gene for mouse and rat and that of Rh-related 50 kD glycoprotein (Rh50) for mouse, rat, and crab-eating macaque. Phylogenetic analyses of Rh and Rh50 amino acid sequences indicate that the Rh50 gene has been evolving about two times more slowly than the Rh blood group gene in both primates and rodents. This conservative nature of the Rh50 gene suggests its relative importance to the Rh blood group gene. The time of gene duplication that produced the Rh and Rh50 genes was estimated to be about 240-310 million years ago. We also conducted window analyses of synonymous and nonsynonymous nucleotide substitutions for those two genes. Some peaks where nonsynonymous substitutions are higher than synonymous ones were located on outer membrane regions. This suggests the existence of positive Darwinian selection on Rh and Rh50 genes through host-parasite interactions.
我们已对小鼠和大鼠的Rh血型基因的完整编码区以及小鼠、大鼠和食蟹猕猴的Rh相关50kD糖蛋白（Rh50）的完整编码区进行了测序。对Rh和Rh50氨基酸序列的系统发育分析表明，在灵长类动物和啮齿动物中，Rh50基因的进化速度比Rh血型基因慢约两倍。Rh50基因的这种保守性质表明了它相对于Rh血型基因的相对重要性。产生Rh和Rh50基因的基因复制时间估计约在2.4亿至3.1亿年前。我们还对这两个基因的同义核苷酸替换和非同义核苷酸替换进行了窗口分析。在外膜区域发现了一些非同义替换高于同义替换的峰值。这表明通过宿主-寄生虫相互作用，Rh和Rh50基因存在正达尔文选择。
