Gon Y, Hashimoto S, Matsumoto K, Nakayama T, Takeshita I, Horie T
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Biochem Biophys Res Commun. 1998 Aug 10;249(1):156-60. doi: 10.1006/bbrc.1998.9115.
p38 mitogen-activated protein kinase (MAP) kinase is activated by various stresses; however, little is known about cold stress which has been shown to cause various inflammatory diseases. In the present study, we examined the effect of cold stimulation on interleukin-8 (IL-8) expression and a role of p38 MAP kinase in IL-8 expression in human bronchial epithelial cells (BEC) in order to clarify the mechanism in hypothermic temperature-induced inflammation. The results showed that cold stimulation induced tyrosine phosphorylation of p38 MAP kinase but not IL-8 expression. IL-8 expression in BEC was induce when the temperature of incubation changed from 1 degree C to 37 degrees C (cooling and rewarming). The specific p38 MAP kinase inhibitor SB 203580 inhibited cooling and rewarming-induced IL-8 expression, indicating that cooling and rewarming-induced IL-8 expression in BEC was mediated through p38 MAP kinase-dependent pathway.
p38丝裂原活化蛋白激酶（MAP激酶）可被多种应激激活；然而，对于已被证明会引发各种炎症性疾病的冷应激却知之甚少。在本研究中，我们检测了冷刺激对白细胞介素-8（IL-8）表达的影响以及p38 MAP激酶在人支气管上皮细胞（BEC）中IL-8表达中的作用，以阐明低温诱导炎症的机制。结果显示，冷刺激诱导了p38 MAP激酶的酪氨酸磷酸化，但未诱导IL-8表达。当孵育温度从1℃变为37℃（冷却和复温）时，BEC中的IL-8表达被诱导。特异性p38 MAP激酶抑制剂SB 203580抑制了冷却和复温诱导的IL-8表达，表明冷却和复温诱导的BEC中IL-8表达是通过p38 MAP激酶依赖性途径介导的。
