雷帕霉素可增强大鼠成骨样骨肉瘤（ROS 17/2.8）细胞的成骨分化。

Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells.

作者信息

Ogawa T, Tokuda M, Tomizawa K, Matsui H, Itano T, Konishi R, Nagahata S, Hatase O

机构信息

Department of Maxillofacial Surgery, Faculty of Medicine, Kagawa Medical University, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Aug 10;249(1):226-30. doi: 10.1006/bbrc.1998.9118.

DOI:10.1006/bbrc.1998.9118

PMID:9705862

Abstract

The effects of three immunosuppressants (rapamycin, FK506 and cyclosporin A) on the proliferation and differentiation of rat osteoblasts-like osteosarcoma cell line, ROS 17/2.8 (ROS) cells were examined in vitro. All immunosuppressants showed a direct inhibition on the proliferation of ROS cells with different potencies. Growth inhibition by rapamycin was stronger than that by FK506 or cyclosporin A. Rapamycin caused a significant increase in alkaline phosphatase (ALP) activity and in the expression of osteopontin and osteocalcin mRNAs. FK506 caused a moderate increase in ALP activity and a decreased expression of osteopontin mRNA. Cyclosporin A caused a decrease in ALP activity and in the expression of type 1 alpha 1 collagen mRNA. Our study indicates that rapamycin directly acts on ROS cells and induces osteoblastic differentiation, however, the effect of FK506 and cyclosporin A is weak. Rapamycin significantly enhances the differentiation induced by 1,25(OH)2-vitaminD3.

摘要

在体外研究了三种免疫抑制剂（雷帕霉素、FK506和环孢素A）对大鼠成骨样骨肉瘤细胞系ROS 17/2.8（ROS）细胞增殖和分化的影响。所有免疫抑制剂均对ROS细胞的增殖表现出不同程度的直接抑制作用。雷帕霉素对细胞生长的抑制作用强于FK506或环孢素A。雷帕霉素可使碱性磷酸酶（ALP）活性以及骨桥蛋白和骨钙素mRNA的表达显著增加。FK506可使ALP活性适度增加，并使骨桥蛋白mRNA的表达降低。环孢素A可使ALP活性以及Ⅰ型α1胶原mRNA的表达降低。我们的研究表明，雷帕霉素直接作用于ROS细胞并诱导成骨细胞分化，然而，FK506和环孢素A的作用较弱。雷帕霉素可显著增强1,25(OH)2-维生素D3诱导的分化作用。