Tsai C C, Kramer S G, Gergen J P
Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794-5215, USA.
Dev Genet. 1998;23(1):35-44. doi: 10.1002/(SICI)1520-6408(1998)23:1<35::AID-DVG4>3.0.CO;2-7.
Ectopic expression of the pair-rule gene runt in the anterior end of the Drosophila embryo antagonizes transcriptional activation of the head gap gene orthodenticle (otd) by the anterior morphogen bicoid. Here we investigate the relevance of runt's activity as a repressor of otd in normal Drosophila embryogenesis otd expression is activated in the posterior region of embryos that are mutant for runt. This posterior expression domain of otd depends on the activity of the orphan nuclear receptor protein Tailless. Repression of otd by runt does not require the conserved VVVRPY motif that mediates interaction between Runt and the co-repressor protein Groucho. The observed functional interactions between runt and tailless on otd expression may indicate there are other contexts where members of these two families of transcriptional regulators interact to regulate gene expression during development.
成对规则基因runt在果蝇胚胎前端的异位表达拮抗了前部形态发生素双胸（bicoid）对头部间隙基因正齿（orthodenticle，otd）的转录激活作用。在此，我们研究了runt作为otd抑制因子在正常果蝇胚胎发育中的活性相关性。在runt突变的胚胎后部区域，otd表达被激活。otd的这个后部表达结构域依赖于孤儿核受体蛋白无尾（Tailless）的活性。runt对otd的抑制作用并不需要介导Runt与共抑制蛋白毛状体（Groucho）相互作用的保守VVVRPY基序。观察到的runt和无尾在otd表达上的功能相互作用可能表明，在发育过程中，这两个转录调节因子家族的成员在其他情况下相互作用以调节基因表达。
