Chang E S, Keller R, Chang S A
Bodega Marine Laboratory, University of California, Bodega Bay, California, 94923, USA.
Gen Comp Endocrinol. 1998 Sep;111(3):359-66. doi: 10.1006/gcen.1998.7120.
An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the lobster, Homarus americanus. It is sensitive to as little as 0.2 fmol of peptide. The assay was used to measure CHH in the hemolymph of intact lobsters after various environmental stresses. Increases in CHH were observed following emersion, exposure to high temperatures (23 degrees and 28 degreesC), and salinity stress (50 and 150% seawater). During emersion, concentrations of hemolymph glucose increased concomitantly with increases in CHH. Significant levels of hemolymph CHH were also measured in lobsters that had been eyestalk-ablated. These latter observations indicate that there may be a source of CHH other than the X-organ/sinus gland in the lobster.
开发了一种用于检测美洲螯龙虾（Homarus americanus）的甲壳类高血糖激素（CHH）的酶联免疫吸附测定法（ELISA）。该方法对低至0.2飞摩尔的肽敏感。该测定法用于测量在各种环境应激后完整龙虾血淋巴中的CHH。在离水、暴露于高温（23摄氏度和28摄氏度）以及盐度应激（50%和150%海水）后，观察到CHH增加。在离水期间，血淋巴葡萄糖浓度随CHH增加而同时升高。在眼柄切除的龙虾中也检测到了显著水平的血淋巴CHH。这些后期观察结果表明，龙虾中可能存在除X器官/窦腺之外的CHH来源。
