乳糜泻患者的小肠T细胞可识别麦醇溶蛋白的天然胃蛋白酶片段。
Small intestinal T cells of celiac disease patients recognize a natural pepsin fragment of gliadin.
作者信息
van de Wal Y, Kooy Y M, van Veelen P A, Peña S A, Mearin L M, Molberg O, Lundin K E, Sollid L M, Mutis T, Benckhuijsen W E, Drijfhout J W, Koning F
机构信息
Department of Immunohaematology and Bloodbank, Leiden University Medical Center, 2300 RC Leiden, The Netherlands.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10050-4. doi: 10.1073/pnas.95.17.10050.
Abstract
Celiac disease is a common severe intestinal disease resulting from intolerance to dietary wheat gluten and related proteins. The large majority of patients expresses the HLA-DQ2 and/or DQ8 molecules, and gluten-specific HLA-DQ-restricted T cells have been found at the site of the lesion in the gut. The nature of peptides that are recognized by such T cells, however, has been unclear so far. We now report the identification of a gliadin-derived epitope that dominantly is recognized by intestinal gluten-specific HLA-DQ8-restricted T cells. The characterization of such epitopes is a key step toward the development of strategies to interfere in mechanisms involved in the pathogenesis of celiac disease.
摘要
乳糜泻是一种常见的严重肠道疾病，由对膳食中的小麦麸质及相关蛋白质不耐受所致。绝大多数患者表达HLA-DQ2和/或DQ8分子，并且在肠道病变部位发现了麸质特异性的HLA-DQ限制性T细胞。然而，这类T细胞所识别的肽的性质迄今尚不清楚。我们现在报告鉴定出一种麦醇溶蛋白衍生的表位，它主要被肠道麸质特异性HLA-DQ8限制性T细胞所识别。此类表位的特性鉴定是开发干预乳糜泻发病机制策略的关键一步。
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Gluten specific, HLA-DQ restricted T cells from coeliac mucosa produce cytokines with Th1 or Th0 profile dominated by interferon gamma.来自乳糜泻黏膜的麸质特异性、HLA - DQ受限的T细胞产生以干扰素γ为主的具有Th1或Th0特征的细胞因子。
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Gliadin-specific, HLA-DQ(alpha 1*0501,beta 1*0201) restricted T cells isolated from the small intestinal mucosa of celiac disease patients.从乳糜泻患者小肠黏膜中分离出的麦醇溶蛋白特异性、HLA - DQ（α1*0501，β1*0201）限制性T细胞。
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Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.培养的人树突状细胞对可溶性抗原的有效呈递可通过粒细胞/巨噬细胞集落刺激因子加白细胞介素4得以维持，而被肿瘤坏死因子α下调。
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