Madhavan H N, Roy S
Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Indian J Ophthalmol. 1998 Mar;46(1):37-40.
The development of mild but significant inflammation probably attributable to viscoelastic ophthalmic solutions in cataract surgery was recently brought to the notice of the authors, and hence a study of the effects of these solutions available in India, on cell cultures was undertaken. We studied the effects of 6 viscoelastic ophthalmic solutions (2 sodium hyaluronate designated as A and B, and 4 hydroxypropylmethylcellulose designated as C, D, E and F) on HeLa, Vero and BHK-21 cell lines in tissue culture microtitre plates using undiluted, 1:10 and 1:100 dilutions of the solutions, and in cover slip cultures using undiluted solutions. Phase contrast microscopic examination of the solutions was also done to determine the presence of floating particles. The products D and F produced cytotoxic changes in HeLa cell line and these products also showed the presence of floating particles under phase contrast microscopy. Other products did not have any adverse effects on the cell lines nor did they show floating particles. The viscoelastic ophthalmic pharmaceutical products designated D and F have cytotoxic effects on HeLa cell line which appears to be a useful cell line for testing these products for their toxicity. The presence of particulate materials in products D and F indicates that the methods used for purification of the solution are not effective.
近期，作者注意到白内障手术中可能由粘弹性眼科溶液引起的轻度但显著的炎症发展情况，因此对印度现有的这些溶液对细胞培养的影响进行了一项研究。我们在组织培养微量滴定板中，使用未稀释、1:10和1:100稀释度的溶液，以及在盖玻片培养中使用未稀释的溶液，研究了6种粘弹性眼科溶液（2种透明质酸钠，分别命名为A和B，以及4种羟丙基甲基纤维素，分别命名为C、D、E和F）对HeLa、Vero和BHK - 21细胞系的影响。还对溶液进行了相差显微镜检查，以确定是否存在漂浮颗粒。产品D和F在HeLa细胞系中产生了细胞毒性变化，并且在相差显微镜下这些产品也显示存在漂浮颗粒。其他产品对细胞系没有任何不良影响，也未显示漂浮颗粒。指定为D和F的粘弹性眼科药品对HeLa细胞系具有细胞毒性作用，HeLa细胞系似乎是用于测试这些产品毒性的有用细胞系。产品D和F中存在颗粒物质表明用于溶液纯化的方法无效。
