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在两种平板培养基上评估大肠杆菌O157:H7在牛肉干中的存活情况。

Survival of Escherichia coli O157:H7 in ground beef jerky assessed on two plating media.

作者信息

Harrison J A, Harrison M A, Rose R A

机构信息

Department of Food and Science and Technology, University of Georgia, Athens 30602, USA.

出版信息

J Food Prot. 1998 Jan;61(1):11-3. doi: 10.4315/0362-028x-61.1.11.

Abstract

Recent outbreaks of food-borne illness due to Salmonella spp. in beef jerky and Escherichia coli O157:H7 in venison jerky, coupled with the fact that a variety of preparation methods and dying procedures abound, raise concern over the safety of processed meat products made in the home. The potential of injured bacterial cells to regain the ability to cause illness is a particular threat with pathogens such as E. coli O157:H7, which is believed to have a low infectious dose. This study examined the efficacy of various methods of jerky preparation in reducing populations of E, coli O157:H7 in ground beef jerky and compared the recovery rate of E. coli O157:H7 on two selective plating media, modified sorbitol MacConkey agar (MSMA) and modified eosin methylene blue agar (MEMB). Populations of E. coli O157:H7 in both heated and unheated samples exhibited a greater decline during drying when a nitrite and salt cure mix was added during jerky preparation. When recovery of E. coli O157:H7 on MSMA and MEMB was compared, a trend toward slightly higher recovery rates with MEMB was observed. On the basis of these results, MEMB is a suitable alternative to MSMA for the recovery of E. coli O157:H7 from heated and dried meat samples similar to beef jerky.

摘要

近期,牛肉干中沙门氏菌引发的食源性疾病疫情,以及鹿肉干中大肠杆菌O157:H7引发的疫情,再加上存在多种制备方法和干燥程序,引发了人们对家庭自制加工肉类产品安全性的担忧。对于像大肠杆菌O157:H7这样据信感染剂量较低的病原体而言,受损细菌细胞恢复致病能力的可能性构成了特别的威胁。本研究检验了各种牛肉干制备方法在降低绞碎牛肉干中大肠杆菌O157:H7数量方面的效果,并比较了大肠杆菌O157:H7在两种选择性平板培养基——改良山梨醇麦康凯琼脂(MSMA)和改良伊红美蓝琼脂(MEMB)上的回收率。当在牛肉干制备过程中添加亚硝酸盐和盐腌制混合物时,加热和未加热样品中大肠杆菌O157:H7的数量在干燥过程中均出现了更大幅度的下降。在比较大肠杆菌O157:H7在MSMA和MEMB上的回收率时,观察到MEMB的回收率略高的趋势。基于这些结果,对于从类似牛肉干的加热和干燥肉类样品中回收大肠杆菌O157:H7而言,MEMB是MSMA的合适替代物。

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