对黄曲霉具有抗性和易感性的玉米基因型种子提取物的蛋白质谱和抗真菌活性

Protein profiles and antifungal activities of kernel extracts from corn genotypes resistant and susceptible to Aspergillus flavus.

作者信息

Guo B Z, Brown R L, Lax A R, Cleveland T E, Russin J S, Widstrom N W

机构信息

Plant Resistance/Germplasm Enhancement Research Unit, Agricultural Research Service, US Department of Agriculture, Tifton, Georgia 31793, USA.

出版信息

J Food Prot. 1998 Jan;61(1):98-102. doi: 10.4315/0362-028x-61.1.98.

DOI:10.4315/0362-028x-61.1.98

PMID:9708260

Abstract

Mechanisms of resistance to infection by the fungus Aspergillus flavus and accumulation of aflatoxin were studied in kernels of resistant (GT-MAS:gk, Mp420) and susceptible ( Pioneer 3154, Deltapine G-4666) corn genotypes. Proteins from kernel extracts of corn genotypes were analyzed by several methods of polyacrylamide gel electrophoresis. Consistent differences in protein profiles were detected among genotypes. Several proteins were unique to or present in greater concentration in resistant genotypes, whereas others were present only in susceptible genotypes. Extracts of resistant kernels showed markedly greater antifungal activity against A. flavus than did susceptible kernel extracts. Results from the present study suggest a role for kernel proteins in resistance to A. flavus infection and aflatoxin contamination in corn genotypes.

摘要

研究了抗黄曲霉感染机制及黄曲霉毒素在抗性（GT-MAS:gk、Mp420）和敏感（先锋3154、三角洲松G-4666）玉米基因型籽粒中的积累情况。采用多种聚丙烯酰胺凝胶电泳方法分析了玉米基因型籽粒提取物中的蛋白质。在不同基因型之间检测到蛋白质谱存在一致差异。几种蛋白质是抗性基因型所特有的或在抗性基因型中浓度更高，而其他蛋白质仅存在于敏感基因型中。抗性籽粒提取物对黄曲霉的抗真菌活性明显高于敏感籽粒提取物。本研究结果表明籽粒蛋白质在玉米基因型对黄曲霉感染和黄曲霉毒素污染的抗性中发挥作用。