Fernandes C F, Flick G J, Thomas T B
Experimental Seafood Processing Laboratory, Mississippi State University, Pascagoula 39567-4156, USA.
J Food Prot. 1998 Mar;61(3):313-7. doi: 10.4315/0362-028x-61.3.313.
Aquacultured rainbow trout (Oncorhynchus mykiss) and channel catfish (Ictalurus punctatus) fillets were inoculated with the psychrotrophic pathogens Listeria monocytogenes and Aeromonas hydrophila: cell populations were monitored during refrigerated storage at 2 to 4 degrees C. Fillets of both species were placed individually in sterile plastic bags and inoculated with cell suspensions (10(4.7) CFU/100 g of fish) of either A. hydrophila or L monocytogenes or of both A. hydrophila and L. monocytogenes, for a total of three treatments for each species of fish. Each inoculum and fillet were mixed to ensure uniform distribution and then stored at 2 to 4 degrees C. A. hydrophila, L. monocytogenes, and aerobic cell populations were determined on days 1, 3, 6, 8, 10, 13, and 15. Individually inoculated A. hydrophila and L. monocytogenes grew on catfish and trout fillets during the 15-day study. There was no inhibition of either pathogen by the natural flora on the fillets. Both psychrotrophic pathogens grew equally well in catfish and trout fillets inoculated with a combination of A. hydrophila and L. monocytogenes. In all three treatments, the counts of the psychrotrophic pathogens were lower than the aerobic plate counts. The growth of the psychrotrophic pathogens L. monocytogenes and/or A. hydrophila during refrigerated storage on aquacultured fish fillets could increase the food hazard risk, particularly where there is a possibility of cross-contamination with ready-to-eat food products.
对养殖虹鳟（Oncorhynchus mykiss）和沟鲶（Ictalurus punctatus）鱼片接种嗜冷性病原菌单核细胞增生李斯特菌和嗜水气单胞菌：在2至4摄氏度冷藏储存期间监测细胞数量。将两种鱼的鱼片分别放入无菌塑料袋中，接种嗜水气单胞菌或单核细胞增生李斯特菌或嗜水气单胞菌与单核细胞增生李斯特菌的细胞悬液（10(4.7) CFU/100 g鱼），每种鱼共三种处理。将每种接种物与鱼片混合以确保均匀分布，然后在2至4摄氏度下储存。在第1、3、6、8、10、13和15天测定嗜水气单胞菌、单核细胞增生李斯特菌和好氧细胞数量。在为期15天的研究中，单独接种的嗜水气单胞菌和单核细胞增生李斯特菌在鲶鱼和虹鳟鱼片上生长。鱼片上的天然菌群对这两种病原菌均无抑制作用。在接种了嗜水气单胞菌和单核细胞增生李斯特菌组合的鲶鱼和虹鳟鱼片中，两种嗜冷性病原菌生长同样良好。在所有三种处理中，嗜冷性病原菌的数量均低于好氧平板计数。嗜冷性病原菌单核细胞增生李斯特菌和/或嗜水气单胞菌在养殖鱼片冷藏储存期间的生长可能会增加食品危害风险，特别是在存在与即食食品交叉污染可能性的情况下。
