Grant M A
Hach Co., Ames, Iowa 50010, USA.
J Food Prot. 1998 Mar;61(3):334-8. doi: 10.4315/0362-028x-61.3.334.
U.S. Food and Drug Administration regulations governing bottled water include microbiological quality guidelines based on coliform counts. Recently, a new MF medium for simultaneous detection of total coliforms and Escherichia coli was developed. This medium, m-ColiBlue24 (m-CB) was compared to m-Endo medium and an International Organization for Standardization standard coliform medium, lactose agar with Tergitol 7. Coliform analysis was conducted on 104 brands of bottled water from 10 countries. Some samples were additionally analyzed for heterotrophic plate count on Pseudomonas sp. populations, including P. aeruginosa. Presumptive coliform colonies were found in 5.8% of the samples with m-CB, 1.9% with m-Endo and 11.5% with lactose agar with Tergitol 7. None of the presumptive coliforms from any of the three media were verified as true coliforms in subsequent analysis. Consequently, the presumptive recovery rates actually represented false-positive error (FPE) rates. The FPE for m-CB and m-Endo were not statistically different (P < 0.05) but the FPE for lactose agar with Tergitol 7 was significantly larger.
美国食品药品监督管理局关于瓶装水的规定包括基于大肠菌群计数的微生物质量指南。最近，一种用于同时检测总大肠菌群和大肠杆菌的新型MF培养基被开发出来。将这种培养基，即m - ColiBlue24（m - CB）与m - Endo培养基以及一种国际标准化组织标准大肠菌群培养基，含聚氧乙烯月桂醚7的乳糖琼脂进行了比较。对来自10个国家的104个品牌的瓶装水进行了大肠菌群分析。一些样品还额外分析了假单胞菌属种群，包括铜绿假单胞菌的异养平板计数。使用m - CB时，5.8%的样品中发现了疑似大肠菌群菌落；使用m - Endo时为1.9%；使用含聚氧乙烯月桂醚7的乳糖琼脂时为11.5%。在后续分析中，来自这三种培养基的任何疑似大肠菌群均未被确认为真正的大肠菌群。因此，疑似回收率实际上代表了假阳性误差（FPE）率。m - CB和m - Endo的FPE在统计学上无差异（P < 0.05），但含聚氧乙烯月桂醚7的乳糖琼脂的FPE显著更高。
