一种用于同时检测和计数大肠杆菌及总大肠菌群的新型膜过滤介质。
A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms.
作者信息
Grant M A
机构信息
Hach Company, Ames, Iowa 50010, USA.
出版信息
Appl Environ Microbiol. 1997 Sep;63(9):3526-30. doi: 10.1128/aem.63.9.3526-3530.1997.
Abstract
Recovery of total coliforms and Escherichia coli on a new membrane filtration (MF) medium was evaluated with 25 water samples from seven states. Testing of the new medium, m-ColiBlue24 broth, was conducted according to a U.S. Environmental Protection Agency protocol. For comparison, this same protocol was used to measure recovery of total coliforms and E. coli with two standard MF media, m-Endo broth and mTEC broth. E. coli recovery on the new medium was also compared to recovery on nutrient agar supplemented with 4-methylumbelliferyl-beta-D-glucuronide. Comparison of specificity, sensitivity, false positive error, undetected target error, and overall agreement indicated E. coli recovery on m-ColiBlue24 was superior to recovery on mTEC for all five parameters. Recovery of total coliforms on the new medium was comparable to recovery on m-Endo.
摘要
使用来自七个州的25份水样评估了一种新型膜过滤（MF）培养基上总大肠菌群和大肠杆菌的回收率。按照美国环境保护局的方案对新型培养基m-ColiBlue24肉汤进行了测试。为作比较，采用相同方案测定了两种标准MF培养基m-远藤肉汤和mTEC肉汤上总大肠菌群和大肠杆菌的回收率。还将新型培养基上大肠杆菌的回收率与补充了4-甲基伞形酮基-β-D-葡萄糖醛酸苷的营养琼脂上的回收率进行了比较。特异性、敏感性、假阳性误差、未检测到目标误差和总体一致性的比较表明，在所有五个参数方面，m-ColiBlue24上大肠杆菌的回收率均优于mTEC上的回收率。新型培养基上总大肠菌群的回收率与m-远藤肉汤上的回收率相当。
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