Castelo M M, Sumner S S, Bullerman L B
Food Science and Technology Department, University of Nebraska-Lincoln 68583-0919, USA.
J Food Prot. 1998 Jun;61(6):704-7. doi: 10.4315/0362-028x-61.6.704.
Corn-based food products obtained from commercial outlets in three different parts of the U.S., Maryland, Nebraska, and Arizona were analyzed for total fumonisins by a commercial competitive direct enzyme-linked immunosorbent assay (CD-ELISA) and for fumonisin B1 (FB1) by high-performance liquid chromatography (HPLC). The highest fumonisin concentrations were found in samples collected in Maryland, where all 18 samples were found positive for fumonisins (200 to 7,450 ng/g of food) by CD-ELISA and 15 of the 18 samples (83%) were found positive for FB1 (< 75 to 5,916 ng/g) by HPLC. Fumonisins were also detected by CD-ELISA in 14 of 15 samples collected in Arizona with concentrations ranging from 200 to 1,450 ng/g, but analyses by HPLC showed that only 8 of 15 samples (53%) were positive for FB1 (< 75 to 1,565 ng/g of food). Of the 23 samples collected in Nebraska, 20 (87%) were positive for fumonisins (200 to 2,500 ng/g) by CD-ELISA, but only 10 (44%) were positive for FB1 (< 75 to 927 ng/g) by HPLC. The highest fumonisin and FB1 concentrations were found in cornmeal samples, ranging up to 7,450 ng/g of cornmeal by CD-ELISA and 5,916 ng/g by HPLC. These findings indicate that there may be a risk of human exposure to fumonisins through the consumption of some corn-based foods.
对从美国三个不同地区（马里兰州、内布拉斯加州和亚利桑那州）的商业销售点获取的玉米基食品进行了分析，通过商业竞争性直接酶联免疫吸附测定（CD - ELISA）检测总伏马毒素，并通过高效液相色谱法（HPLC）检测伏马毒素B1（FB1）。在马里兰州采集的样本中发现伏马毒素浓度最高，通过CD - ELISA检测，所有18个样本的伏马毒素均呈阳性（食品中含量为200至7450 ng/g），通过HPLC检测，18个样本中有15个（83%）的FB1呈阳性（含量<75至5916 ng/g）。在亚利桑那州采集的15个样本中，有14个通过CD - ELISA检测出伏马毒素，浓度范围为200至1450 ng/g，但HPLC分析表明，15个样本中只有8个（53%）的FB1呈阳性（食品中含量<75至1565 ng/g）。在内布拉斯加州采集的23个样本中，20个（87%）通过CD - ELISA检测伏马毒素呈阳性（含量为200至2500 ng/g），但通过HPLC检测，只有10个（44%）的FB1呈阳性（含量<75至927 ng/g）。伏马毒素和FB1的最高浓度出现在玉米粉样本中，通过CD - ELISA检测，玉米粉中伏马毒素含量高达7450 ng/g，通过HPLC检测为5916 ng/g。这些发现表明，通过食用某些玉米基食品，人类可能有接触伏马毒素的风险。
