Daniel G B, DeNovo R, Schultze A E, Schmidt D, Smith G T
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.
Vet Radiol Ultrasound. 1998 Jul-Aug;39(4):375-83. doi: 10.1111/j.1740-8261.1998.tb01623.x.
The extraction of the hepatobiliary radiopharmaceutical 99mTc-mebrofenin (Choletec) by the liver can be used to evaluate the severity of hepatocellular disease. The hepatic parenchymal cells extract mebrofenin from the blood by the same active transport mechanism as bilirubin. The ability of the liver to extract 99mTc-mebrofenin is a measure of hepatic parenchymal cell function. In this study, we induced hepatocellular disease by administration of a hepatotoxic drug and compared a direct method of determining the hepatic extraction of 99mTc-mebrofenin to hepatic extraction fraction derived from deconvolutional analysis. We also compared both methods of calculating the hepatic extraction of 99mTc-mebrofenin to liver histopathology. Hepatic extraction fraction derived from deconvolutional analysis correlated very well to the direct measurement technique (R=0.922, p < 0.001). Both methods of determining hepatic extraction correlated well to quantitative histopathology, having the same correlation coefficient and p values. (R=-0.833, p=0.003). As the hepatic extraction 99mTc-mebrofenin decreased, the severity of the histopathologic lesions of the liver increased in a linear fashion. There was a significant correlation of the hepatic excretion T1/2 to quantitative histopathology (R=0.949, p < 0.001). The hepatic excretion T1/2 increased as the severity of the histopathologic lesions of the liver increased. Hepatic extraction (HEF) and excretion of 99mTc-mebrofenin are good predictors of the severity of hepatocellular damage in toxic induced liver disease. This study helps validate the premise that HEF derived from deconvolutional analysis is a good predictor of the actual first pass hepatic extraction of 99mTc-mebrofenin.
肝脏对肝胆放射性药物99mTc-美布芬宁（胆影脒）的摄取可用于评估肝细胞疾病的严重程度。肝实质细胞通过与胆红素相同的主动转运机制从血液中摄取美布芬宁。肝脏摄取99mTc-美布芬宁的能力是肝实质细胞功能的一种衡量指标。在本研究中，我们通过给予肝毒性药物诱导肝细胞疾病，并将直接测定99mTc-美布芬宁肝脏摄取的方法与去卷积分析得出的肝脏摄取分数进行比较。我们还将计算99mTc-美布芬宁肝脏摄取的两种方法与肝脏组织病理学进行比较。去卷积分析得出的肝脏摄取分数与直接测量技术相关性非常好（R=0.922，p<0.001）。两种确定肝脏摄取的方法与定量组织病理学相关性都很好，具有相同的相关系数和p值（R=-0.833，p=0.003）。随着99mTc-美布芬宁肝脏摄取的降低，肝脏组织病理学损伤的严重程度呈线性增加。肝脏排泄半衰期与定量组织病理学有显著相关性（R=0.949，p<0.001）。随着肝脏组织病理学损伤严重程度的增加，肝脏排泄半衰期延长。99mTc-美布芬宁的肝脏摄取（HEF）和排泄是中毒性肝病中肝细胞损伤严重程度的良好预测指标。本研究有助于验证以下前提，即去卷积分析得出的HEF是99mTc-美布芬宁实际首次通过肝脏摄取的良好预测指标。
