Rodrigo J, Fernández P, Bentura M L, de Velasco J M, Serrano J, Uttenthal O, Martínez-Murillo R
Department of Comparative Neuroanatomy, Instituto Cajal, C.S.I.C., Madrid, Spain.
J Chem Neuroanat. 1998 Jul;15(1):1-20. doi: 10.1016/s0891-0618(98)00016-7.
The topographical distribution of catecholaminergic nerve fibres and their anatomical relationship to cholinergic elements in the rat globus pallidus were studied. Peroxidase-antiperoxidase and two-colour immunoperoxidase staining procedures were used to demonstrate tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT) and choline acetyltransferase (ChAT) immunoreactivities, combined with acetylcholinesterase (AChE) pharmacohistochemistry. TH immunoreactive nerve fibres were seen to enter the globus pallidus from the medial forebrain bundle. The greatest density of such fibres was found in the ventral region of the globus pallidus, which was also characterized by the greatest density of ChAT immunoreactive neurons. TH immunoreactive nerve fibres showed varicose arborizations and sparse boutons, which were occasionally seen in close opposition to cholinergic structures. In all regions of the globus pallidus, there were also larger, smooth TH immunoreactive nerve fibres of passage to the caudate putamen. A smaller number of DBH immunoreactive nerve fibres and terminal arborizations were found in the substantia innominata, internal capsule and in the globus pallidus bordering these structures. A few PNMT immunoreactive nerve fibres in the substantia innominata and internal capsule did not enter the globus pallidus. Electron microscopy revealed TH immunoreactive synaptic profiles in the ventromedial area of the globus pallidus corresponding to the nucleus basalis magnocellularis of Meynert (nBM). These made mainly symmetrical and only a few asymmetrical synaptic contacts with dendrites containing AChE reaction product. The results indicate that cholinergic structures in the nBM are innervated by dopaminergic fibres and terminals, with only a very small input from noradrenergic fibres.
研究了大鼠苍白球中儿茶酚胺能神经纤维的拓扑分布及其与胆碱能元件的解剖关系。采用过氧化物酶 - 抗过氧化物酶和双色免疫过氧化物酶染色程序来显示酪氨酸羟化酶（TH）、多巴胺β-羟化酶（DBH）、苯乙醇胺N-甲基转移酶（PNMT）和胆碱乙酰转移酶（ChAT）的免疫反应性，并结合乙酰胆碱酯酶（AChE）药物组织化学。可见TH免疫反应性神经纤维从内侧前脑束进入苍白球。此类纤维的最大密度见于苍白球腹侧区域，该区域也是ChAT免疫反应性神经元密度最大的区域。TH免疫反应性神经纤维呈现曲张分支和稀疏的终扣，偶尔可见其与胆碱能结构紧密相邻。在苍白球的所有区域，也有较大的、光滑的TH免疫反应性神经纤维通向尾壳核。在无名质、内囊以及与这些结构相邻的苍白球中发现了少量DBH免疫反应性神经纤维和终末分支。无名质和内囊中少数PNMT免疫反应性神经纤维未进入苍白球。电子显微镜显示，苍白球腹内侧区域存在与迈内特基底大细胞核（nBM）相对应的TH免疫反应性突触轮廓。这些突触主要与含有AChE反应产物的树突形成对称突触联系，仅有少数不对称突触联系。结果表明，nBM中的胆碱能结构由多巴胺能纤维和终末支配，去甲肾上腺素能纤维的输入非常少。
