Okasha K, Yang H, Shoker A S
Department of Medicine, Royal University Hospital, Saskatoon, Saskatchewan, Canada.
Clin Nephrol. 1998 Jul;50(1):21-7.
This study was designed to investigate the presence of IgG1 alloreactive memory cells in the peripheral blood in humans and their in vitro activation requirements. Alloreactive antibody production was measured after cell activation with cytokines: Interleukin (IL) 2, IL-4 and IL-10, interferon gamma, alloantigens and/or OKT3, lipopolysaccharide or Pokeweed mitogen and Epstein-Barr virus transformation. The examined cells were taken from ten sensitized and five nonsensitized uremic patients with previous graft loss and five normal controls. The titers and percentage panel reactive IgG1 antibody reactivity present in the respective sera was further compared with the in vitro profile. Alloreactive antibody reactivity was measured by PRA-STAT ELISA method. The results show that: 1) Short term control T cell lines or nonsensitized cells were unable to provide the necessary help to autologous B cells to produce alloreactive antibodies of the IgG subclass. 2) Activated cells from sensitized patients produced low levels of alloreactive IgGl antibodies. 3) Stimulation with any of the cytokines and/or mitogens or alloantigens or allopeptides was not sufficient to produce consistent levels of alloreactive IgG 1 antibodies, in spite of its presence in the respective sera.
本研究旨在调查人类外周血中IgG1同种异体反应性记忆细胞的存在情况及其体外激活需求。在用细胞因子（白细胞介素（IL）-2、IL-4和IL-10、干扰素γ、同种异体抗原和/或OKT3）、脂多糖或商陆有丝分裂原以及爱泼斯坦-巴尔病毒转化激活细胞后，测量同种异体反应性抗体的产生。所检测的细胞取自10名致敏且之前有移植物丢失的尿毒症患者、5名未致敏的尿毒症患者以及5名正常对照。将各血清中存在的滴度和群体反应性IgG1抗体反应性百分比进一步与体外情况进行比较。通过PRA-STAT ELISA方法测量同种异体反应性抗体反应性。结果显示：1）短期对照T细胞系或未致敏细胞无法为自体B细胞提供产生IgG亚类同种异体反应性抗体所需的帮助。2）致敏患者的活化细胞产生低水平的同种异体反应性IgG1抗体。3）尽管在各血清中存在同种异体反应性IgG1抗体，但用任何一种细胞因子和/或有丝分裂原或同种异体抗原或异源肽进行刺激都不足以产生一致水平的同种异体反应性IgG1抗体。
