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从产生肿瘤病毒的人细胞系中消除一种副粘病毒型病毒(作者译)

[Elimination of a paramyxo-type virus from oncornavirus-producing human cell lines (author's transl)].

作者信息

Niezabitowski A, Bender E, Micheel B, Rudolph M, Wunderlich V, Widmaier R, Papsdorf G, Sydow G, Uckert W, Bierwolf D, Graffi A

出版信息

Arch Geschwulstforsch. 1976;46(3):187-96.

PMID:971040
Abstract

A virus of the paramyxo-type was eliminated from cell-free material of human oncornavirus-producing cell lines (PMF). After transmission of this paramyxovirus-free inoculum to a human permanent cell strain (Tu 197/Tr 1) oncornaviruses were permanently formed and no paramyxoviruses could be detected. The paramyxovirus-free, oncornavirus-producing PMF-39 cell line could be established after inoculation of the TU 197/Tr 1 line with cell-free material containing both oncorna- and paramyxovirus diluted 1 to 1000. A second way of elimination of the paramyxovirus was the treatment of cell-free material containing both viruses with antisera against paramyxovirus. In the Tu 197/Tr 1 line inoculated with such material only oncornaviruses were formed. The second paramyxovirus-free oncornavirus-producing cell line was designated PMF 50.

摘要

一种副粘病毒型病毒从产生人肿瘤病毒的细胞系(PMF)的无细胞材料中被清除。将这种不含副粘病毒的接种物接种到一种人类永久细胞株(Tu 197/Tr 1)后,肿瘤病毒被永久形成,且未检测到副粘病毒。在用含有按1:1000稀释的肿瘤病毒和副粘病毒的无细胞材料接种TU 197/Tr 1细胞系后,可建立不含副粘病毒、产生肿瘤病毒的PMF - 39细胞系。清除副粘病毒的另一种方法是用抗副粘病毒抗血清处理含有两种病毒的无细胞材料。在用这种材料接种的Tu 197/Tr 1细胞系中,仅形成了肿瘤病毒。第二个不含副粘病毒、产生肿瘤病毒的细胞系被命名为PMF 50。

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