评估三维晶体中膜蛋白的功能。
Assessing the functionality of a membrane protein in a three-dimensional crystal.
作者信息
Heberle J, Büldt G, Koglin E, Rosenbusch J P, Landau E M
机构信息
Institut für Biologische Informationsverarbeitung IBI-2: Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany.
出版信息
J Mol Biol. 1998 Aug 28;281(4):587-92. doi: 10.1006/jmbi.1998.1970.
PMID:9710532
Abstract
Hexagonal microcrystals of bacteriorhodopsin embedded in a lipidic cubic phase have been investigated by time-resolved FT-IR and resonance Raman spectroscopy. Retinal isomerization, conformational changes in the protein backbone and proton translocation are virtually indistinguishable from those in the native membrane. The protein is thus fully active in three-dimensional crystals.
摘要
嵌入脂质立方相的细菌视紫红质的六角形微晶已通过时间分辨傅里叶变换红外光谱和共振拉曼光谱进行了研究。视黄醛异构化、蛋白质主链的构象变化和质子转运与天然膜中的情况几乎无法区分。因此，该蛋白质在三维晶体中具有完全活性。
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