Numa Y, Tsukazaki Y, Yamamoto M, Matsudaira T, Kawamoto K
Dept. of Neurosurgery Kansai Medical University, Osaka, Japan.
Hum Cell. 1998 Mar;11(1):21-6.
Cyclins are key components of the cell cycle progression machinery. Immunohistochemical detection of cyclins in relation to cell position, DNA content, by multiparameter flow cytometry has provided new cell cycle studies. T98G, U373MG, and U251MG were scheduled expression of cyclin A, B1 which are maximal in late S and G2M phase cells. T98G was scheduled expression of cyclin E. being maximal in G1 and S phase cells, but U251MG, U373MG were unscheduled expression of cyclin E. The expression of cyclin D1 were not expressed a pattern. These result may mean that cyclin E in an important protein of cell cycle machinery of malignant brain tumor.
细胞周期蛋白是细胞周期进程机制的关键组成部分。通过多参数流式细胞术对细胞周期蛋白与细胞位置、DNA含量进行免疫组织化学检测，为新的细胞周期研究提供了依据。T98G、U373MG和U251MG细胞中细胞周期蛋白A、B1呈定时表达，在S期末期和G2M期细胞中表达量最高。T98G细胞中细胞周期蛋白E呈定时表达，在G1期和S期细胞中表达量最高，但U251MG和U373MG细胞中细胞周期蛋白E呈非定时表达。细胞周期蛋白D1未呈现出特定的表达模式。这些结果可能意味着细胞周期蛋白E是恶性脑肿瘤细胞周期机制中的一种重要蛋白质。
