Kumagai N
Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki-shi, Japan.
Hum Cell. 1998 Mar;11(1):61-8.
The author have done clinical application of cultured epithelium grafting since 1985. In this article, the results of auto- and allografting (patients: 365 cases) were reported. By the cultivation of epidermal cells, many amount of cultured epithelium could be obtained. Cultured autografts took well to the partial thickness wounds. Grafted sites healed promptly and histologically resembled normal skin. Disfigurement on the skin surfaces was well treated and improved by autografting. Full thickness wounds caused by burn or excision of congenital giant nevi were treated with cryopreserved allogeneic skin and fresh autologous cultured epithelium. The results showed good graft take and histology. Allogeneic cultured epithelium accelerated the wound healing on the grafted area. Thus, the autologous and allogeneic cultured epithelial grafting procedure is a promising treatment for many patients suffering from large skin wounds or disfigurement of skin surfaces.
自1985年以来，作者开展了培养上皮移植的临床应用。本文报告了自体移植和异体移植（患者365例）的结果。通过表皮细胞培养，可获得大量培养上皮。培养的自体移植物能很好地贴合于部分厚度伤口。移植部位愈合迅速，组织学上类似正常皮肤。皮肤表面的毁容通过自体移植得到了良好治疗和改善。由烧伤或先天性巨痣切除引起的全层伤口，采用冷冻保存的同种异体皮肤和新鲜自体培养上皮进行治疗。结果显示移植效果良好且组织学表现良好。异体培养上皮加速了移植区域的伤口愈合。因此，自体和异体培养上皮移植手术对于许多患有大面积皮肤伤口或皮肤表面毁容的患者来说是一种很有前景的治疗方法。
