Taneda A, Shindoh C, Ohuchi Y, Shirato K
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1998 May;185(1):45-54. doi: 10.1620/tjem.185.45.
The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline + endotoxin group as control and an IL-10 + endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline + endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p < 0.001 and p < 0.05, respectively) compared to those at 0 hour. In the IL-10 + endotoxin group, the decrement in the force-frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline + endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-10 + endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.
利用Wistar大鼠研究了内毒素给药后静脉注射白细胞介素（IL）-10对膈肌的影响。动物被分为两个治疗组：生理盐水+内毒素组作为对照组和IL-10+内毒素组。在注射生理盐水或IL-10（1250 U，0.25 mg）5分钟后，腹腔注射大肠杆菌内毒素（10 mg/kg）。在内毒素注射后0小时和4小时测量力-频率曲线、抽搐动力学和疲劳性。在生理盐水+内毒素组，与0小时相比，4小时时力-频率曲线和半松弛时间显著降低（分别为p<0.001和p<0.05）。在IL-10+内毒素组，与0小时相比，4小时时内毒素引起的力-频率曲线下降得到预防。烟酰胺腺嘌呤二核苷酸磷酸（NADPH）黄递酶组织化学显示，生理盐水+内毒素组在4小时时呈阳性染色，但IL-10+内毒素组在0小时或4小时时无明显染色。这些数据表明，IL-10通过抑制膈肌中一氧化氮的产生来预防内毒素诱导的收缩恶化。
