Nijbroek G L, Michaelis S
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Methods Enzymol. 1998;292:193-212. doi: 10.1016/s0076-6879(98)92016-x.
As a member of the ABC superfamily, STE6 is unique in that it has a well-characterized substrate, a-factor, and can be easily manipulated in the yeast system. Functional assays have been extensively used, and methods to examine trafficking and stability of STE6 are well established. In addition, STE6 chimeras and ste6 deletion strains are useful for the analysis of many nonyeast ABC proteins. Continuing studies of STE6 are expected to aid in the identification of novel cellular components involved in the trafficking and functioning of not only STE6, but of other members of the ABC superfamily as well.
作为ABC超家族的一员，STE6具有独特之处，即它有一个特征明确的底物——a因子，并且在酵母系统中易于操作。功能分析已被广泛应用，检测STE6转运和稳定性的方法也已成熟。此外，STE6嵌合体和ste6缺失菌株对于分析许多非酵母ABC蛋白很有用。对STE6的持续研究有望有助于识别不仅参与STE6，而且参与ABC超家族其他成员转运和功能的新型细胞成分。
