取代半胱氨酸可及性方法。 - Suppr

Suppr

超能文献

Substituted-cysteine accessibility method.

作者信息

Karlin A, Akabas M H

机构信息

Department of Biochemistry, Columbia University College of Physicians, New York, New York 10032-3702, USA.

出版信息

Methods Enzymol. 1998;293:123-45. doi: 10.1016/s0076-6879(98)93011-7.

DOI:10.1016/s0076-6879(98)93011-7

PMID:9711606

Abstract

摘要

相似文献

Substituted-cysteine accessibility method.取代半胱氨酸可及性方法。

Methods Enzymol. 1998;293:123-45. doi: 10.1016/s0076-6879(98)93011-7.

Exposure of residues in the cyclic nucleotide-gated channel pore: P region structure and function in gating.环核苷酸门控通道孔内残基的暴露：门控过程中的P区结构与功能

Neuron. 1996 Jan;16(1):141-9. doi: 10.1016/s0896-6273(00)80031-8.

Identification of channel-lining residues in the M2 membrane-spanning segment of the GABA(A) receptor alpha1 subunit.γ-氨基丁酸A（GABA(A)）受体α1亚基M2跨膜片段中通道内衬残基的鉴定

J Gen Physiol. 1996 Feb;107(2):195-205. doi: 10.1085/jgp.107.2.195.

Selected cysteine residues in transmembrane domains of mu-opioid receptor are critical for effects of sulfhydryl reagents.μ-阿片受体跨膜结构域中选定的半胱氨酸残基对巯基试剂的作用至关重要。

J Pharmacol Exp Ther. 2000 Apr;293(1):113-20.

Contribution of the beta subunit M2 segment to the ion-conducting pathway of the acetylcholine receptor.β亚基M2片段对乙酰胆碱受体离子传导途径的贡献。

Biochemistry. 1998 Jun 2;37(22):7952-64. doi: 10.1021/bi980143m.

Change of pore helix conformational state upon opening of cyclic nucleotide-gated channels.环核苷酸门控通道开放时孔螺旋构象状态的变化。

Neuron. 2000 Dec;28(3):899-909. doi: 10.1016/s0896-6273(00)00162-8.

Channel-lining residues of the AMPA receptor M2 segment: structural environment of the Q/R site and identification of the selectivity filter.AMPA受体M2片段的通道内衬残基：Q/R位点的结构环境及选择性过滤器的鉴定

J Neurosci. 2001 Jun 15;21(12):4162-72. doi: 10.1523/JNEUROSCI.21-12-04162.2001.

Evidence for the proximity of the extreme N-terminus of the neurokinin-2 (NK2) tachykinin receptor to Cys167 in the putative fourth transmembrane helix.神经激肽-2（NK2）速激肽受体的极端N端与假定的第四跨膜螺旋中的半胱氨酸167接近的证据。

Biochemistry. 2004 Mar 23;43(11):3027-38. doi: 10.1021/bi035475s.

The mitochondrial oxoglutarate carrier: cysteine-scanning mutagenesis of transmembrane domain IV and sensitivity of Cys mutants to sulfhydryl reagents.线粒体α-酮戊二酸载体：跨膜结构域IV的半胱氨酸扫描诱变及半胱氨酸突变体对巯基试剂的敏感性

Biochemistry. 2001 Dec 25;40(51):15805-10. doi: 10.1021/bi011616j.

Site-directed mutagenesis and chemical modification of the six native cysteine residues of the rat mitochondrial carnitine carrier: implications for the role of cysteine-136.大鼠线粒体肉碱载体六个天然半胱氨酸残基的定点诱变和化学修饰：对半胱氨酸-136作用的启示

Biochemistry. 2002 Jul 9;41(27):8649-56. doi: 10.1021/bi012183n.

引用本文的文献

Structural and Functional Mechanisms Underlying Activation Gate Dynamics and IFM Motif Accessibility in Human Na1.5.人类Na1.5中激活门控动力学和IFM模体可及性的结构与功能机制

bioRxiv. 2025 Jul 4:2025.06.29.662239. doi: 10.1101/2025.06.29.662239.

Interaction of unphosphorylated PtsN with the K/H antiporter YcgO inhibits its activity in Escherichia coli.未磷酸化的PtsN与钾/氢反向转运蛋白YcgO之间的相互作用会抑制其在大肠杆菌中的活性。

J Biol Chem. 2025 Feb;301(2):108153. doi: 10.1016/j.jbc.2024.108153. Epub 2024 Dec 30.

A pore locus in the E1 domain differentially regulates Cx26 and Cx30 hemichannel function.E1 结构域中的一个孔位差异调节 Cx26 和 Cx30 半通道功能。

J Gen Physiol. 2024 Nov 4;156(11). doi: 10.1085/jgp.202313502. Epub 2024 Sep 20.

The divisome is a self-enhancing machine in Escherichia coli and Caulobacter crescentus.分裂酶是大肠杆菌和新月柄杆菌中的一种自我增强机器。

Nat Commun. 2024 Sep 18;15(1):8198. doi: 10.1038/s41467-024-52217-5.

Elevator-like movements of prestin mediate outer hair cell electromotility.耳鼓肌蛋白的电梯样运动介导了外毛细胞的电运动。

Nat Commun. 2023 Nov 6;14(1):7145. doi: 10.1038/s41467-023-42489-8.

Exploring Uniform, Dual, and Dynamic Topologies of Membrane Proteins by Substituted Cysteine Accessibility Method (SCAM™).通过取代半胱氨酸可及性方法（SCAM™）探索膜蛋白的均匀、双重和动态拓扑结构。

Methods Mol Biol. 2024;2715:121-157. doi: 10.1007/978-1-0716-3445-5_9.

Substituted Cysteine Modification and Protection with n-Alkyl-MTS Reagents Quantifies Steric Changes Induced by a Mutation in Anesthetic Binding Sites on GABA Type A Receptors.用 n- 烷基-MTS 试剂进行取代半胱氨酸修饰和保护，定量分析 GABA 型 A 受体麻醉结合位点突变引起的空间变化。

Mol Pharmacol. 2023 Dec;104(6):266-274. doi: 10.1124/molpharm.123.000719. Epub 2023 Aug 16.

The roles of two extracellular loops in proton sensing and permeation in human Otop1 proton channel.人类 Otop1 质子通道中两个细胞外环在质子感应和渗透中的作用。

Commun Biol. 2022 Oct 20;5(1):1110. doi: 10.1038/s42003-022-04085-2.

How Does Our Knowledge on the Na/H Exchanger NHE1 Obtained by Biochemical and Molecular Analyses Keep up With Its Recent Structure Determination?通过生化和分子分析获得的关于钠/氢交换体NHE1的知识如何跟上其最近的结构测定？

Front Physiol. 2022 Jul 15;13:907587. doi: 10.3389/fphys.2022.907587. eCollection 2022.

Acinetobacter baumannii Can Survive with an Outer Membrane Lacking Lipooligosaccharide Due to Structural Support from Elongasome Peptidoglycan Synthesis.鲍曼不动杆菌可以在外膜缺乏脂寡糖的情况下存活，这是由于延长体肽聚糖合成提供的结构支持。

mBio. 2021 Dec 21;12(6):e0309921. doi: 10.1128/mBio.03099-21. Epub 2021 Nov 30.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验