Siegrist C A, Saddallah F, Tougne C, Martinez X, Kovarik J, Lambert P H
WHO Collaborating Centre for Neonatal Vaccinology, Department of Pathology, University of Geneva, Switzerland.
Vaccine. 1998 Aug-Sep;16(14-15):1473-8. doi: 10.1016/s0264-410x(98)00111-x.
Failure to generate CTL responses in early life has been linked to the preferential maturation of CD4 T cells into TH2 rather than TH1 cells in response to some, but not other, antigenic stimulations. Here, we provide preliminary evidence for the role of the viral replication pattern in the shaping of neonatal cellular responses to live viral vaccines. Neonatal and early life immunization with live attenuated Sendai virus vaccine led to the induction of IgG2a antibodies and cytotoxic responses as efficiently as immunization of adult animals. Similarly, although early life immunization with live attenuated measles virus led to preferential TH2 polarization of T cells compared with adult primed animals, it allowed the induction of CTL responses which had not been observed following immunization with a live recombinant canarypox vector. Thus, conversely to a non-replicating canarypox recombinant vaccine expressing the measles haemagglutonin, viral vaccines with limited but present replication capacity appear capable of activating neonatal antigen presenting cells to trigger TH1 and CTL responses, as recently observed for DNA vaccines.
早年无法产生细胞毒性T淋巴细胞（CTL）反应，与某些（而非其他）抗原刺激下CD4 T细胞优先成熟为TH2细胞而非TH1细胞有关。在此，我们提供了初步证据，证明病毒复制模式在塑造新生儿对活病毒疫苗的细胞反应中所起的作用。用减毒活仙台病毒疫苗对新生儿和早年动物进行免疫，与对成年动物进行免疫一样有效地诱导了IgG2a抗体和细胞毒性反应。同样，虽然与成年初免动物相比，用减毒活麻疹病毒对早年动物进行免疫导致T细胞优先向TH2极化，但它能够诱导出在用重组金丝雀痘病毒活载体免疫后未观察到的CTL反应。因此，与表达麻疹血凝素的非复制型金丝雀痘重组疫苗相反，具有有限但存在的复制能力的病毒疫苗似乎能够激活新生儿抗原呈递细胞，以触发TH1和CTL反应，正如最近在DNA疫苗中所观察到的那样。
