Bagley M J, Gall G A
Department of Animal Science, University of California, Davis 95616, USA.
Mol Ecol. 1998 Aug;7(8):945-61.
Mitochondrial and nuclear DNA variability was examined to assess population genetic structure and phylogeographic relationships in rainbow trout. Single-strand conformation polymorphisms and restriction site differences within 1055 bp of the mitochondrial D-loop region and 1566 bp of nuclear DNA in six single-copy nuclear DNA regions identified 31 mitochondrial genotypes and 50 nuclear alleles. Gene trees were constructed by sequencing each variant allele or mitochondrial genotype identified. Examination of 30 populations in 10 native rainbow trout groups using analysis of molecular variance (AMOVA) indicated that 65% of mitochondrial variability and 35% of nuclear variability was explained by differences among the 10 groups. Phylogenetic patterns evident in mitochondrial and nuclear DNA were not always concordant. Differences in the evolutionary patterns detected by mitochondrial and nuclear DNA may reflect the differential impact of past introgression events on variability in the two genomes.
研究了线粒体和核DNA的变异性，以评估虹鳟鱼的种群遗传结构和系统发育地理关系。对线粒体D环区域1055 bp和六个单拷贝核DNA区域1566 bp的核DNA内的单链构象多态性和限制性位点差异进行分析，确定了31种线粒体基因型和50个核等位基因。通过对每个鉴定出的变异等位基因或线粒体基因型进行测序构建基因树。使用分子方差分析（AMOVA）对10个本地虹鳟鱼群体中的30个种群进行检测，结果表明，10个群体之间的差异解释了65%的线粒体变异性和35%的核变异性。线粒体和核DNA中明显的系统发育模式并不总是一致的。线粒体和核DNA检测到的进化模式差异可能反映了过去基因渗入事件对两个基因组变异性的不同影响。
