Watanabe N
Department of Laboratory Diagnosis, Sapporo Medical University, School of Medicine, Japan.
Leuk Lymphoma. 1998 Aug;30(5-6):477-82. doi: 10.3109/10428199809057560.
Sensitivity to doxorubicin (DOX) and expression of intracellular endogenous TNF (enTNF) or manganous superoxide dismutase (MnSOD) activity were found to be inversely correlated in leukemic cells from 19 patients (6 acute lymphoblastic leukemia, 13 acute myeloblastic leukemia). In a case with acquired resistance to chemotherapy which included DOX, enTNF expression and MnSOD activity were increased. Furthermore, in 14 cases treated with a regimen including an anthracycline, 4 cases which failed to respond to chemotherapy showed relatively high amounts of enTNF expression. After TNF antisense cDNA was transfected into leukemic cells isolated from 5 patients, sensitivity of transfectants to DOX increased 1.4- to 2.5-fold. Overall, we found enTNF to act as a resistance factor against DOX in leukemia which may predict response to DOX.
在19例白血病患者（6例急性淋巴细胞白血病，13例急性髓细胞白血病）的白血病细胞中，发现对阿霉素（DOX）的敏感性与细胞内内源性肿瘤坏死因子（enTNF）的表达或锰超氧化物歧化酶（MnSOD）活性呈负相关。在1例对包括DOX在内的化疗产生获得性耐药的病例中，enTNF表达和MnSOD活性增加。此外，在14例接受含蒽环类药物方案治疗的病例中，4例化疗无效的病例显示enTNF表达量相对较高。将TNF反义cDNA转染到5例患者分离出的白血病细胞中后，转染子对DOX的敏感性提高了1.4至2.5倍。总体而言，我们发现enTNF在白血病中作为对抗DOX的耐药因子，可能预测对DOX的反应。
