Guerassimov A, Zhang Y, Banerjee S, Cartman A, Webber C, Esdaile J, Fitzcharles M A, Poole A R
Joint Diseases Laboratory, Shriners Hospital for Children, McGill University, Montreal, Quebec, Canada.
J Rheumatol. 1998 Aug;25(8):1480-4.
To determine whether patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS) express cellular immunity to cartilage link protein (LP).
LP was purified from human fetal epiphyseal and bovine adult nasal cartilage. It was used in proliferation assays with the peripheral blood lymphocytes (PBL) isolated from 83 patients with RA, 21 patients with AS, and 30 healthy controls.
Patients with RA (34%) and AS (71%) expressed a significantly higher prevalence of cellular immune responses to human LP compared with the healthy control group (13%). Such significant differences were not observed for bovine LP. Half the patients with RA responding to LP exhibited cellular immunity to both human and bovine protein. In the AS group, PBL from a majority of responders to LP recognized only human LP.
These data suggest that LP is a potential autoantigen in the development of RA and AS and that cellular immune reactivity to common and distinct LP epitopes in patients with RA and AS may play a role in the pathogenesis of these diseases.
确定类风湿关节炎（RA）和强直性脊柱炎（AS）患者是否对软骨连接蛋白（LP）产生细胞免疫。
从人胎儿骨骺和牛成年鼻软骨中纯化LP。将其用于对从83例RA患者、21例AS患者和30名健康对照者分离出的外周血淋巴细胞（PBL）进行增殖试验。
与健康对照组（13%）相比，RA患者（34%）和AS患者（71%）对人LP产生细胞免疫反应的患病率显著更高。对于牛LP未观察到此类显著差异。对LP有反应的RA患者中有一半对人和牛蛋白均表现出细胞免疫。在AS组中，大多数对LP有反应者的PBL仅识别出人LP。
这些数据表明LP是RA和AS发病过程中的一种潜在自身抗原，并且RA和AS患者对常见和不同LP表位的细胞免疫反应性可能在这些疾病的发病机制中起作用。
