Lauwers G Y, Grant L D, Scott G V, Carr N J, Sobin L H
Department of Pathology and Laboratory Medicine, University of Florida College of Medicine, Gainesville 32610-0275, USA.
Hum Pathol. 1998 Aug;29(8):863-8. doi: 10.1016/s0046-8177(98)90458-0.
Spindle cell squamous esophageal carcinomas are distinctive polypoid "biphasic" tumors in which the sarcoma-like phenotype usually predominates over the epithelial component. To biologically assess both phenotypes, we compared the tumoral proliferative activity and DNA ploidy between the two histological components of 13 polypoid spindle cell squamous carcinomas of the esophagus. We studied the tumoral proliferative index (TPI) using MIB 1 monoclonal antibody (Ki-67) and determined the DNA histogram by image cytometry on Feulgen-stained sections. The DNA histograms were classified into four types (I to IV) according to the degree of dispersion of the DNA. The TPI of the carcinomatous regions ranged from 0.20 to 0.63 (mean, 0.44) and from 0.55 to 0.85 for the sarcoma-like areas (mean, 0.68) P < .0001. In all cases, the sarcoma-like areas were aneuploid, and 37.5% of the carcinomatous regions were diploid. Also, in all instances the carcinomatous areas were of either histogram type I or II, and the sarcoma-like areas showed histograms of type II or III. We conclude that in esophageal spindle cell squamous carcinomas the sarcoma-like phenotype differs biologically in two ways from the carcinomatous: (1) it has a higher TPI and (2) it has higher aneuploidy with a greater dispersion of the DNA content. We postulate that these characteristics could give a "growth" advantage to the sarcoma-like component of these tumors and explain its predominance over the carcinomatous component.
梭形细胞鳞状食管癌是一种独特的息肉样“双相”肿瘤，其中肉瘤样表型通常比上皮成分占优势。为了从生物学角度评估这两种表型，我们比较了13例食管息肉样梭形细胞鳞状癌的两个组织学成分之间的肿瘤增殖活性和DNA倍体。我们使用MIB 1单克隆抗体（Ki-67）研究肿瘤增殖指数（TPI），并通过对福尔根染色切片进行图像细胞术测定DNA直方图。根据DNA的分散程度，将DNA直方图分为四种类型（I至IV）。癌性区域的TPI范围为0.20至0.63（平均0.44），肉瘤样区域为0.55至0.85（平均0.68），P <.0001。在所有病例中，肉瘤样区域为非整倍体，37.5%的癌性区域为二倍体。此外，在所有情况下，癌性区域为I型或II型直方图，而肉瘤样区域显示为II型或III型直方图。我们得出结论，在食管梭形细胞鳞状癌中，肉瘤样表型在生物学上与癌性表型有两个不同之处：（1）它具有更高的TPI；（2）它具有更高的非整倍体性，DNA含量的分散性更大。我们推测这些特征可能赋予这些肿瘤的肉瘤样成分“生长”优势，并解释其比癌性成分占优势的原因。
