Ratnaraj N, Patsalos P N
University Department of Clinical Neurology, Institute of Neurology, London, United Kingdom.
Ther Drug Monit. 1998 Aug;20(4):430-4. doi: 10.1097/00007691-199808000-00013.
A gradient high-performance liquid chromatography micromethod is described for the simultaneous quantitation of vigabatrin and gabapentin in human serum. Chromatography was performed using a 125- x 3-mm ID Hypersil BDS C-18 column with a 3-microm mini-bore, eluted with a gradient system comprised of phosphate buffer (pH 6.5)-acetonitrile-methanol-water at a flow rate of 0.45 ml/minute. The column eluent was monitored on a fluorescence detector using excitation and emission wavelengths of 340 and 440 nm, respectively. The lower limit of quantitation for vigabatrin and for gabapentin was 5 micromol/l, and the within-batch and between-batch coefficients of variation were <5%. No interference from commonly prescribed antiepileptic drugs (carbamazepine and its metabolite carbamazepine epoxide, oxcarbazepine and its metabolite 10-hydroxycarbazepine, ethosuximide, lamotrigine, phenobarbitone, phenytoin, primidone, and valproic acid) was observed; thus, the method can be used to monitor vigabatrin and gabapentin in patients on polytherapy antiepileptic drug regimens.
本文描述了一种梯度高效液相色谱微方法，用于同时定量测定人血清中的氨己烯酸和加巴喷丁。色谱分析使用内径为125×3 mm的Hypersil BDS C-18柱，带有3微米的微径柱，以由磷酸盐缓冲液（pH 6.5）-乙腈-甲醇-水组成的梯度系统洗脱，流速为0.45 ml/分钟。在荧光检测器上监测柱洗脱液，激发波长和发射波长分别为340和440 nm。氨己烯酸和加巴喷丁的定量下限为5 μmol/l，批内和批间变异系数<5%。未观察到常用抗癫痫药物（卡马西平及其代谢产物卡马西平环氧化物、奥卡西平及其代谢产物10-羟基卡马西平、乙琥胺、拉莫三嗪、苯巴比妥、苯妥英、扑米酮和丙戊酸）的干扰；因此，该方法可用于监测接受联合抗癫痫药物治疗方案的患者体内的氨己烯酸和加巴喷丁。
