Mitsuhashi W, Saito H, Sato M, Nakashima N, Noda H
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan.
Virus Res. 1998 May;55(1):61-9. doi: 10.1016/s0168-1702(98)00034-3.
The complete nucleotide sequence of the spheroidin gene of Anomala cuprea entomopoxvirus (AcEPV) was determined. The sequence was compared with those of spheroidin genes of EPVs such as Melolontha melolontha EPV (MmEPV, Genus A), Choristoneura fumiferana EPV (CfEPV, Genus B) and Amsacta moorei EPV (AmEPV, Genus B). The gene harbored an open reading frame (ORF) of 2826 nt, with the same size as that of MmEPV belonging to the same genus, capable of coding for a polypeptide of 109.0 kDa. The predicted amino acid (aa) sequence showed a greater or moderate similarity to the corresponding sequence of the other EPVs, showing a 94, 40 and 41% aa identity with MmEPV, CfEPV and AmEPV, respectively; the identity was 89, 53 and 54% at the nucleotide level. The hydropathy plots also showed a greater similarity in organization to MmEPV and moderate similarity to the viruses of Genus B. In the polypeptide, 44 cysteine residues, which are likely to be involved in paracrystal formation and seven potential N-glycosylation sites were detected. The number of cysteine residues and N-glycosylation sites also depended on the difference in genera (A or B). Thus, the spheroidin gene of EPVs was well conserved within the same genus.
测定了铜绿异丽金龟昆虫痘病毒（AcEPV）球状体蛋白基因的完整核苷酸序列。将该序列与其他昆虫痘病毒（EPV）的球状体蛋白基因序列进行了比较，如五月鳃金龟EPV（MmEPV，A属）、云杉芽卷蛾EPV（CfEPV，B属）和摩尔夜蛾EPV（AmEPV，B属）。该基因含有一个2826 nt的开放阅读框（ORF），与同属的MmEPV大小相同，能够编码一个109.0 kDa的多肽。预测的氨基酸（aa）序列与其他EPV的相应序列显示出较高或中等程度的相似性，与MmEPV、CfEPV和AmEPV的氨基酸同一性分别为94%、40%和41%；在核苷酸水平上的同一性分别为89%、53%和54%。亲水性图谱也显示出与MmEPV在结构上有较高的相似性，与B属病毒有中等程度的相似性。在该多肽中，检测到44个可能参与副晶体形成的半胱氨酸残基和7个潜在的N-糖基化位点。半胱氨酸残基和N-糖基化位点的数量也取决于属（A或B）的差异。因此，EPV的球状体蛋白基因在同一属内保守性良好。
