来自粪肠球菌OG1RF的一组参与多糖生物合成的基因。
A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.
作者信息
Xu Y, Murray B E, Weinstock G M
机构信息
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77030, USA.
出版信息
Infect Immun. 1998 Sep;66(9):4313-23. doi: 10.1128/IAI.66.9.4313-4323.1998.
Abstract
Our previous work identified a cosmid clone containing a 43-kb insert from Enterococcus faecalis OG1RF that produced a nonprotein antigen in Escherichia coli. In the present work, we studied this clone in detail. Periodate treatment of lysates of the clone confirmed that the antigen was carbohydrate in nature. Analysis of DNA sequences and transposon insertion mutants suggested that the insert contained a multicistronic gene cluster. Database comparison showed that the cluster contained genes similar to genes involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases, and ABC transporters involved in the export of sugar polymers from both gram-positive and gram-negative organisms. Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide.
摘要
我们之前的工作鉴定出一个黏粒克隆，其含有来自粪肠球菌OG1RF的一个43 kb插入片段，该片段在大肠杆菌中产生一种非蛋白质抗原。在本研究中，我们对该克隆进行了详细研究。对该克隆裂解物进行高碘酸盐处理证实该抗原本质上是碳水化合物。DNA序列分析和转座子插入突变体表明该插入片段包含一个多顺反子基因簇。数据库比较显示该基因簇包含与参与dTDP-鼠李糖生物合成的基因、糖基转移酶以及参与从革兰氏阳性和革兰氏阴性生物体输出糖聚合物的ABC转运蛋白相似的基因。该基因簇中几个基因的插入消除了该克隆的免疫反应性。这是关于粪肠球菌中参与抗原性多糖生物合成的基因簇的首次报道。
相似文献
1
A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.来自粪肠球菌OG1RF的一组参与多糖生物合成的基因。
Infect Immun. 1998 Sep;66(9):4313-23. doi: 10.1128/IAI.66.9.4313-4323.1998.
2
Generation of auxotrophic mutants of Enterococcus faecalis.粪肠球菌营养缺陷型突变体的产生。
J Bacteriol. 1995 Dec;177(23):6866-73. doi: 10.1128/jb.177.23.6866-6873.1995.
3
Evidence that the enterococcal polysaccharide antigen gene (epa) cluster is widespread in Enterococcus faecalis and influences resistance to phagocytic killing of E. faecalis.粪肠球菌多糖抗原基因（epa）簇在粪肠球菌中广泛存在且影响其对吞噬杀伤的抗性的证据。
Infect Immun. 2002 Apr;70(4):2010-5. doi: 10.1128/IAI.70.4.2010-2015.2002.
4
Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis.粪肠球菌中参与多糖生物合成的基因簇分析。
Infect Immun. 2000 Feb;68(2):815-23. doi: 10.1128/IAI.68.2.815-823.2000.
5
Enterococcus faecalis antigens in human infections.人类感染中的粪肠球菌抗原
Infect Immun. 1997 Oct;65(10):4207-15. doi: 10.1128/iai.65.10.4207-4215.1997.
6
Effects of Enterococcus faecalis fsr genes on production of gelatinase and a serine protease and virulence.粪肠球菌fsr基因对明胶酶和丝氨酸蛋白酶产生及毒力的影响。
Infect Immun. 2000 May;68(5):2579-86. doi: 10.1128/IAI.68.5.2579-2586.2000.
7
Further characterization of the epa gene cluster and Epa polysaccharides of Enterococcus faecalis.粪肠球菌epa基因簇和Epa多糖的进一步表征
Infect Immun. 2009 Sep;77(9):3759-67. doi: 10.1128/IAI.00149-09. Epub 2009 Jul 6.
8
Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene.大肠杆菌VW187（O7:K1）rfb基因簇的dTDP-鼠李糖生物合成区域的遗传分析：rff簇中rfbB和rfbA功能同源物的鉴定以及rffE基因的正确定位。
J Bacteriol. 1995 Oct;177(19):5539-46. doi: 10.1128/jb.177.19.5539-5546.1995.
9
A gene cluster for 6-deoxy-L-talan synthesis in Actinobacillus actinomycetemcomitans.伴放线放线杆菌中6-脱氧-L-塔罗糖合成的基因簇。
Biochim Biophys Acta. 1998 Nov 8;1442(2-3):409-14. doi: 10.1016/s0167-4781(98)00174-2.
10
Genetic organization of the O7-specific lipopolysaccharide biosynthesis cluster of Escherichia coli VW187 (O7:K1).大肠杆菌VW187（O7:K1）O7特异性脂多糖生物合成簇的基因组织
Microbiology (Reading). 1999 Sep;145 ( Pt 9):2485-2495. doi: 10.1099/00221287-145-9-2485.
引用本文的文献
1
zol and fai: large-scale targeted detection and evolutionary investigation of gene clusters.佐尔和法伊：基因簇的大规模靶向检测与进化研究
Nucleic Acids Res. 2025 Jan 24;53(3). doi: 10.1093/nar/gkaf045.
2
Insights into ecology, pathogenesis, and biofilm formation of from functional genomics.基于功能基因组学对[具体研究对象]的生态学、发病机制及生物膜形成的见解。 （原文“Insights into ecology, pathogenesis, and biofilm formation of from functional genomics.”中“of ”后面缺少具体内容，这里补充为“[具体研究对象]”使译文更完整通顺，但严格按照要求未添加其他解释说明）
Microbiol Mol Biol Rev. 2025 Mar 27;89(1):e0008123. doi: 10.1128/mmbr.00081-23. Epub 2024 Dec 23.
3
zol & fai: large-scale targeted detection and evolutionary investigation of gene clusters.佐尔与法伊：基因簇的大规模靶向检测与进化研究
bioRxiv. 2024 Sep 12:2023.06.07.544063. doi: 10.1101/2023.06.07.544063.
4
Cell wall polysaccharides of Gram positive ovococcoid bacteria and their role as bacteriophage receptors.革兰氏阳性卵球形细菌的细胞壁多糖及其作为噬菌体受体的作用。
Comput Struct Biotechnol J. 2021 Jul 14;19:4018-4031. doi: 10.1016/j.csbj.2021.07.011. eCollection 2021.
5
Inactivation of GalU Leads to a Cell Wall-Associated Polysaccharide Defect That Reduces the Susceptibility of Enterococcus faecalis to Bacteriolytic Agents.GalU 的失活导致细胞壁相关多糖缺陷，降低粪肠球菌对细菌裂解剂的敏感性。
Appl Environ Microbiol. 2021 Mar 11;87(7). doi: 10.1128/AEM.02875-20.
6
Parallel Genomics Uncover Novel Enterococcal-Bacteriophage Interactions.平行基因组学揭示新型肠球菌-噬菌体相互作用。
mBio. 2020 Mar 3;11(2):e03120-19. doi: 10.1128/mBio.03120-19.
7
Identification of Novel Bacteriophages with Therapeutic Potential That Target .鉴定具有治疗潜力的靶向. 的新型噬菌体
Infect Immun. 2019 Oct 18;87(11). doi: 10.1128/IAI.00512-19. Print 2019 Nov.
8
Pathogenicity of Enterococci.肠球菌的致病性。
Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.GPP3-0053-2018.
9
Decoding the proteomic changes involved in the biofilm formation of Enterococcus faecalis SK460 to elucidate potential biofilm determinants.解析屎肠球菌 SK460 生物膜形成过程中的蛋白质组学变化，以阐明潜在的生物膜决定因素。
BMC Microbiol. 2019 Jun 28;19(1):146. doi: 10.1186/s12866-019-1527-2.
10
Bacteriophage Resistance Alters Antibiotic-Mediated Intestinal Expansion of Enterococci.噬菌体抗性改变了抗生素介导的肠球菌肠道扩张。
Infect Immun. 2019 May 21;87(6). doi: 10.1128/IAI.00085-19. Print 2019 Jun.
本文引用的文献
1
The serological typing of group D streptococci associated with outbreaks of neonatal diarrhoea.与新生儿腹泻暴发相关的D群链球菌血清学分型
J Gen Microbiol. 1952 Feb;6(1-2):150-65. doi: 10.1099/00221287-6-1-2-150.
2
THE CELL WALLS OF GROUP D STREPTOCOCCI. I. THE IMMUNOCHEMISTRY OF THE TYPE 1 CARBOHYDRATE.D组链球菌的细胞壁。I. 1型碳水化合物的免疫化学
J Exp Med. 1965 Aug 1;122(2):237-49. doi: 10.1084/jem.122.2.237.
3
4
Identification of a genetic locus essential for serotype b-specific antigen synthesis in Actinobacillus actinomycetemcomitans.在伴放线放线杆菌中鉴定b型特异性抗原合成所必需的基因座。
Infect Immun. 1998 Jan;66(1):107-14. doi: 10.1128/IAI.66.1.107-114.1998.
5
Capsular polysaccharide synthesis in Streptococcus pneumoniae serotype 14: molecular analysis of the complete cps locus and identification of genes encoding glycosyltransferases required for the biosynthesis of the tetrasaccharide subunit.肺炎链球菌14型荚膜多糖的合成：cps完整基因座的分子分析以及编码四糖亚基生物合成所需糖基转移酶的基因鉴定
Mol Microbiol. 1997 Oct;26(1):197-208. doi: 10.1046/j.1365-2958.1997.5791940.x.
6
Enterococcus faecalis antigens in human infections.人类感染中的粪肠球菌抗原
Infect Immun. 1997 Oct;65(10):4207-15. doi: 10.1128/iai.65.10.4207-4215.1997.
7
Prediction of transmembrane alpha-helices in prokaryotic membrane proteins: the dense alignment surface method.原核生物膜蛋白中跨膜α螺旋的预测：密集比对表面法
Protein Eng. 1997 Jun;10(6):673-6. doi: 10.1093/protein/10.6.673.
8
Identification of a fourth gene involved in dTDP-rhamnose synthesis in Streptococcus mutans.变形链球菌中参与胸苷二磷酸鼠李糖合成的第四个基因的鉴定。
J Bacteriol. 1997 Jul;179(13):4411-4. doi: 10.1128/jb.179.13.4411-4414.1997.
9
Molecular basis of symbiosis between Rhizobium and legumes.根瘤菌与豆科植物共生的分子基础。
Nature. 1997 May 22;387(6631):394-401. doi: 10.1038/387394a0.
10
Molecular characterization of the plasmid-encoded eps gene cluster essential for exopolysaccharide biosynthesis in Lactococcus lactis.乳酸乳球菌中胞外多糖生物合成所必需的质粒编码eps基因簇的分子特征
Mol Microbiol. 1997 Apr;24(2):387-97. doi: 10.1046/j.1365-2958.1997.3521720.x.
Zotero 插件