Dobiasova M, Kymla J, Faltova E
Atherosclerosis. 1976 Sep;24(3):421-9. doi: 10.1016/0021-9150(76)90134-9.
Rats were injected intravenously with liposomes made of [4(-14)C] cholesterol with [32P]lysolecithin, or [4(-14)C]cholesterol with [32P]lecithin. The clearance of both radioactive labels from plasma was observed, as well as their distribution in the organs after 15 and 60 min. At the same time, the esterification of injected [14C]cholesterol and the conversion of [32P]lysolecithin to [32P]lecithin and vice versa were examined. [14C]Cholesterol administered with lysolecithin was cleared from the plasma at a higher rate than with lecithin. Consequently the radioactivity of [14C]cholesterol in the aorta, heart, lung, kidney and liver changed with the applied phospholipid; with lysolecithin it was higher than with lecithin. Lysolecithin itself was distributed among the organs more evenly than lecithin, which accumulated most in the liver. If administered with lysolecithin, [14C]cholesterol was esterified in the plasma in a significantly higher proportion than if administered with lecithin. The antiatherogenous effect of lecithin and the atherogenous effect of lysolecithin are considered on the basis of different transport properties of these phospholipids.
给大鼠静脉注射由[4(-14)C]胆固醇与[32P]溶血卵磷脂制成的脂质体，或[4(-14)C]胆固醇与[32P]卵磷脂制成的脂质体。观察了两种放射性标记物从血浆中的清除情况，以及15分钟和60分钟后它们在各器官中的分布。同时，检测了注射的[14C]胆固醇的酯化情况以及[32P]溶血卵磷脂向[32P]卵磷脂的转化情况，反之亦然。与卵磷脂相比，与溶血卵磷脂一起给予的[14C]胆固醇从血浆中的清除率更高。因此，主动脉、心脏、肺、肾和肝脏中[14C]胆固醇的放射性随所应用的磷脂而变化；与溶血卵磷脂一起时比与卵磷脂一起时更高。溶血卵磷脂本身在各器官中的分布比卵磷脂更均匀，卵磷脂在肝脏中积累最多。如果与溶血卵磷脂一起给予，[14C]胆固醇在血浆中的酯化比例比与卵磷脂一起给予时显著更高。基于这些磷脂不同的转运特性，对卵磷脂的抗动脉粥样硬化作用和溶血卵磷脂的促动脉粥样硬化作用进行了探讨。
