A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass.
作者信息
Rosoff J D, Soltow L O, Vocelka C R, Schmer G, Chandler W L, Cochran R P, Kunzelman K S, Spiess B D
机构信息
Department of Anesthesiology, University of Washington, Seattle 98195, USA.
出版信息
J Cardiothorac Vasc Anesth. 1998 Aug;12(4):397-401. doi: 10.1016/s1053-0770(98)90191-4.
OBJECTIVES
To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production.
DESIGN
A prospective, randomized, single-blinded, ex vivo model.
SETTING
A university hospital, laboratory, and clinics.
PARTICIPANTS
Ten healthy adult consented volunteer blood donors (five men, five women).
INTERVENTIONS
Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood.
MEASUREMENTS AND MAIN RESULTS
Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone.
CONCLUSION
The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.
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