第二代血液替代品（全氟二氯辛烷乳剂）在体外循环旁路模型中不会激活补体。
A second-generation blood substitute (perfluorodichlorooctane emulsion) does not activate complement during an ex vivo circulation model of bypass.
作者信息
Rosoff J D, Soltow L O, Vocelka C R, Schmer G, Chandler W L, Cochran R P, Kunzelman K S, Spiess B D
机构信息
Department of Anesthesiology, University of Washington, Seattle 98195, USA.
出版信息
J Cardiothorac Vasc Anesth. 1998 Aug;12(4):397-401. doi: 10.1016/s1053-0770(98)90191-4.
OBJECTIVES
To examine whether a second-generation perfluorocarbon (PFC) blood substitute added to the cardiopulmonary bypass (CPB) prime influences complement production.
DESIGN
A prospective, randomized, single-blinded, ex vivo model.
SETTING
A university hospital, laboratory, and clinics.
PARTICIPANTS
Ten healthy adult consented volunteer blood donors (five men, five women).
INTERVENTIONS
Ex vivo closed-loop extracorporeal circuit including membrane oxygenator, tubing, and filter primed with crystalloid or crystalloid plus PFC was circulated for 1 hour with the addition of 500 mL of heparinized fresh human whole blood.
MEASUREMENTS AND MAIN RESULTS
Laboratory specimens were drawn from the circuit at 10-minute intervals for 1 hour and measured for complement (C3a, Bb fragment) concentrations, blood gases, fibrinogen concentration, platelet count, and hematocrit. In the PFC group, C3a and Bb fragments were equal to or less than those in the group that received crystalloid alone.
CONCLUSION
The second-generation PFC added to the prime of a CPB circuit does not independently increase complement production.
目的
研究添加到体外循环（CPB）预充液中的第二代全氟碳（PFC）血液代用品是否会影响补体生成。
设计
前瞻性、随机、单盲体外模型。
地点
大学医院、实验室和诊所。
参与者
10名健康成年自愿献血者（5名男性，5名女性）。
干预措施
体外闭环体外循环，包括膜式氧合器、管道和用晶体液或晶体液加PFC预充的过滤器，加入500 mL肝素化新鲜人全血后循环1小时。
测量指标和主要结果
每隔10分钟从循环回路中采集实验室标本，持续1小时，测量补体（C3a、Bb片段）浓度、血气、纤维蛋白原浓度、血小板计数和血细胞比容。在PFC组中，C3a和Bb片段等于或低于仅接受晶体液的组。
结论
添加到CPB回路预充液中的第二代PFC不会独立增加补体生成。
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