Yu Z, Karem K L, Kanangat S, Manickan E, Rouse B T
Immunology Department, Mayo Clinic, Rochester, MN 55905, USA.
Vaccine. 1998 Oct;16(17):1660-7. doi: 10.1016/s0264-410x(98)00065-6.
To test the principle that genetically engineered epitopes in a plasmid DNA can efficiently induce specific immunity, a minigene cassette encoding cytotoxic T lymphocyte (CTL), helper T and B cell epitopes from herpes simplex virus (HSV) was constructed and placed in an expression vector named pcMini. Following immunizations with pcMini, mice developed epitope-specific CTLs comparable to the response induced by live HSV. Less effective but detectable antibody, lymphoproliferation, and T cell cytokine responses were also produced. In addition, pcMini-primed mice elicited a recall response upon restimulation with recombinant vaccinia virus expressing HSV antigen. The protection provided by minigene vaccination was significant, although not as efficient as live virus vaccine. The DNA minigene approach may prove useful to define and induce immune responses against minimal antigenic determinants.
为了验证质粒DNA中的基因工程表位能有效诱导特异性免疫这一原理，构建了一个编码来自单纯疱疹病毒（HSV）的细胞毒性T淋巴细胞（CTL）、辅助性T细胞和B细胞表位的小基因盒，并将其置于名为pcMini的表达载体中。用pcMini免疫小鼠后，小鼠产生了与活HSV诱导的反应相当的表位特异性CTL。还产生了效果较差但可检测到的抗体、淋巴细胞增殖和T细胞细胞因子反应。此外，用表达HSV抗原的重组痘苗病毒再次刺激时，经pcMini预免疫的小鼠引发了回忆反应。小基因疫苗提供的保护作用显著，尽管不如活病毒疫苗有效。DNA小基因方法可能被证明有助于定义和诱导针对最小抗原决定簇的免疫反应。
