Fuchs N, Sonda S, Gottstein B, Hemphill A
Institute of Parasitology of the Faculty of Veterinary Medicine, University of Berne, Switzerland.
J Parasitol. 1998 Aug;84(4):753-8.
Morphologically, the tachyzoites and the tissue cysts of Neospora caninum are difficult to distinguish from those of other cyst-forming apicomplexan parasites such as Toxoplasma gondii. Several stage-specific antigens have been identified in T. gondii tachyzoites and bradyzoites, and respective antibodies are useful tools for discriminating between the 2 stages during tachyzoite-bradyzoite interconversion in T. gondii infections. Whereas several cell surface- and dense granule-associated proteins have been identified and characterized in N. caninum tachyzoites, not much is known about antigenic components expressed in N. caninum bradyzoites. In this study, the differential expression of the 2 N. caninum surface proteins Nc-p43 and Nc-p36 and the dense granule protein Nc-p33 (NCDG1) within tachyzoites and bradyzoites of N. caninum has been investigated.
从形态学上看，犬新孢子虫的速殖子和组织包囊很难与其他形成包囊的顶复门寄生虫（如刚地弓形虫）的区分开来。在刚地弓形虫的速殖子和缓殖子中已鉴定出几种阶段特异性抗原，相应的抗体是区分刚地弓形虫感染中速殖子-缓殖子相互转化过程中这两个阶段的有用工具。虽然在犬新孢子虫速殖子中已鉴定并表征了几种细胞表面和致密颗粒相关蛋白，但对犬新孢子虫缓殖子中表达的抗原成分了解甚少。在本研究中，已对犬新孢子虫速殖子和缓殖子中两种犬新孢子虫表面蛋白Nc-p43和Nc-p36以及致密颗粒蛋白Nc-p33（NCDG1）的差异表达进行了研究。
