Makioka A, Ohtomo H, Kobayashi S, Takeuchi T
Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan.
J Parasitol. 1998 Aug;84(4):857-9.
We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 microg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.
我们之前已经证明，溶组织内阿米巴的DNA聚合酶活性受到阿非迪霉素的抑制，阿非迪霉素是真核细胞核复制性DNA聚合酶的特异性抑制剂。本研究旨在评估阿非迪霉素对该寄生虫生长和DNA合成的影响。阿非迪霉素阻断了无菌溶组织内阿米巴菌株HM-1:IMSS的生长。当通过将[3H]胸苷掺入DNA来测定时，阿非迪霉素也抑制了DNA合成。去除药物后，阿非迪霉素对溶组织内阿米巴生长的抑制作用被消除，并且暴露于3微克/毫升的药物中至少48小时对其活力影响很小。去除阿非迪霉素后的恢复阶段观察到同步生长。
